Environmental laboratories a CEPT

Laboratory for Genetically Modified Animals

                   LABORATORY FOR GENETICALLY                

MODIFIED ANIMALS

 

Head:

Piotr P. Kosson, PhD

This email address is being protected from spambots. You need JavaScript enabled to view it.

tel. 22 6086454

Staff:

tech. Łukasz Puchalski – tel. 22 6066433

Michał Kwiatkowski tel. 22 6066433

 

The Laboratory for Genetically Modified Animals was created to conduct research on the models of animals and offers:

- breading genetically modified animals

- maintenance of animals during experiments

- in vivo scientific research with the use of animal models consistent with the protocol of Ethics Committee

 

Furthermore the laboratory offers:

-technical advice in the design of research (selection of animal model, valuation of the number of  animals, the submission of the application to the Local Ethical Committee

- marking, collecting samples for genetic analyses,  genotyping consistent with protocol

- small surgery

- collecting samples for analyses

- substances administration (iv, ip, im, it, po)

- immunisation of animals

- euthanasia of animals using mixture of CO2 and O2

 

The laboratory is focused on cooperating with:

- scientific institutions  within  the framework of scientific cooperation and implementation research and development of projects

- companies and consortia of companies and scientific units conducting research of pharmacokinetics and pharmacodynamics of new drug therapies

Main equipment1. Breeding room:- individually ventilated cages system (IVC)- cage changing station 2. Surgery room:- rodent anesthesia apparatus- dual configuration of cabinet: microbiological safety and cage changing- IVC recovery unit3. Washing room4. Water treatment station for rodents

  

Main equipment

1. Breeding room:

- individually ventilated cages system (IVC)

- cage changing station

 

2. Surgery room:

- rodent anesthesia apparatus

- dual configuration of cabinet: microbiological safety and cage changing

- IVC recovery unit

3. Washing room

4. Water treatment station for rodents

The most important parameters:

- barrier-conditioned system, entry through air shower

 - area of breeding rooms 2 x 36 m2

- area of surgery room 10 m2

- temp. 220 C +/- 20 C

- humidity 55% +/- 5%

- atmospheric pressure regulated by ventilation and air-conditioning systems

- bedding  (sterile chips from deciduous/coniferous trees)

- complete, standard feed for rodents, granulated, sterilized with gamma rays

  

 

The laboratory carries  out the following research projects:

- the study of biological activity and therapeutic effect of drugs in the model of asthma induced by HDM or OVA, in pulmonary fibrosis model induced by bleomycin and in the model of colitis induced by DSS (the study covered by confidentiality)

- vaccine against flu – innovative solution for obtaining subunits of antigens, NCBiR PBS2/A7/14/2014, (the study covered by confidentiality)

- evaluation of the effectiveness of different variants of HA antigen obtained in yeast cells within the project “Centre of medicinal product biotechnology. Package of innovative biopharmaceuticals for human and animal therapy and prophylactics.”   POIG.01.01.02.-14-007/08-06 European Funds Portal Innovative Economy (the study covered by confidentiality)

 

The laboratory carries out the following mice breading projects:

-KO and TG: BS-K 707, YAC-128, Shiverer, RagSOD

 

The laboratory conducts research in cooperation with:

 
 

Price list

 Customer contracting service bears the costs of exploitation of equipment and maintenance of one animal per every day of the maintenance (fixed fee). Due to the nature of laboratory work (work for sanitary barrier) the access to the laboratory is limited to the laboratory workers and specially trained people, which has influence on the final price where fixed fee cost is added to  future services such as genotyping, small surgery, collecting biological samples, technical advice in the planning of the experiment and the submission of the application to the Local Ethics Committee

 

Recent publications:

Kopera E, Zdanowski K,2, Uranowska K, Kosson P, Sączyńska V, Florys K, Szewczyk B. High-Titre Neutralizing Antibodies to H1N1 Influenza Virus after Mouse Immunization with Yeast Expressed H1 Antigen: A Promising Influenza Vaccine Candidate. J Immunol Res. 2019 Jan 8;2019:2463731

Motyl J, Przykaza Ł, Boguszewski PM, Kosson P, Strosznajder JB. Pramipexole and Fingolimod exert neuroprotection in a mouse model of Parkinson's disease by activation of sphingosine kinase 1 and Akt kinase. Neuropharmacology. 2018 Jun;135:139-150.

Czeredys M, Vigont VA, Boeva VA, Mikoshiba K, Kaznacheyeva EV, Kuznicki J. Huntingtin-Associated Protein 1A Regulates Store-Operated Calcium Entry in Medium Spiny Neurons From Transgenic YAC128 Mice, a Model of Huntington's Disease. Front Cell Neurosci. 2018 Oct 26;12:381

Redkiewicz P, Stachyra A, Sawicka RA, Bocian K, Góra-Sochacka A, Kosson P, Sirko A. Immunogenicity of DNA Vaccine against H5N1 Containing Extended Kappa B Site: In Vivo Study in Mice and Chickens. Front Immunol. 2017 Aug 24;8:1012

Macioła AK, Pietrzak MA, Kosson P, Czarnocki-Cieciura M, Śmietanka K, Minta Z, Kopera E. The Length of N-Glycans of Recombinant H5N1 Hemagglutinin Influences the Oligomerization and Immunogenicity of Vaccine Antigen. Front Immunol. 2017 Apr 20;8:444.

Stachyra A, Redkiewicz P, Kosson P, Protasiuk A, Góra-Sochacka A, Kudla G, Sirko A. Codon optimization of antigen coding sequences improves the immune potential of DNA vaccines against avian influenza virus H5N1 in mice and chickens. Virol J. 2016 Aug 26;13(1):143