NeuroRepair Department

NeuroRepair Department

Head   Joanna Sypecka, Prof, PhD, phone: +48 22 60 86 510; 60 86 508,This email address is being protected from spambots. You need JavaScript enabled to view it.

Secretariat      Krystyna Wasik, phone: +48 22 60 86 510  This email address is being protected from spambots. You need JavaScript enabled to view it.

Research staff

  • Teresa Zalewska, Prof. Ph.D.; D.Sc. -phone: +48 22 60 86 532; This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Barbara Lukomska, Prof. Ph.D.;D.Sc.-phone: +48 22 60 86 448; This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Malgorzata Nalecz, Ph.D.;D.Sc. - phone: +48 22 60 86 529; This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Miroslaw Janowski, MD; Ph.D.; D.Sc. - phone: +48 22 60 86 629; This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Luiza Stanaszek, Ph.D. – phone +48 22 60 86 529; This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Katarzyna Drela Ph.D.- phone +48 22 60 86 529; This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Anna Andrzejewska, Ph.D.- phone +48 22 60 86 471; This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Sylwia Dabrowska, Ph.D.- phone +48 22 60 86 471;  This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Justyna Janowska, M.Sc., phone +48 22 60 86 471: This email address is being protected from spambots. You need JavaScript enabled to view it.

Technical staff

  • Zajac Halina, phone + 48 22 60 86 531

PhD students

  • Gargas Justyna, M.Sc., phone +48 22 60 86 618, This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Pawelec Paulina, M.Sc., phone  +48 22 60 86 618, This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Rogujski Piotr, M.Sc., phone  +48 22 60 86 529, This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Ziabska Karolina, M.Sc., phone  +48 22 60 86 618, This email address is being protected from spambots. You need JavaScript enabled to view it.

Graduate students

  • Araszkiewicz Antonina (UW), phone + 48 22 608 64 71
  • Stachowicz Patrycja (UW), phone 22 608 64 71

Research profile

The research interest is focused on:

  • human mesenchymal stem cells derived from bone marrow and cell microvesicles in term of their isolation, identification, expansion, genetic modifications and therapeutic transplantation in animal models of brain ischemia
  • interpretation of the role the histone acetylation/deacetylation play in stimulating the endogenous neurogenesis in rat model of perinatal asphyxia
  • evaluation of the selected mechanisms of neuron-glia and glia-glia interactions during CNS neurodegeneration and neurorepair
  • description of temporary ischemia-hypoxia influence on the oligodendrocyte progenitor maturation and process of CNS myelination
  • effectiveness of glial progenitor transplantation in the treatment of ALS disease in the experimental models (rag2/shi; rag2/SOD1mice)

Grants

  • “Involvement of endo- and exogenous factors in neuroreparative processes evolved by the CNS damage” (2020-2021), Statutory project no 6, Principal investigator-Joanna Sypecka
  • “The alterations in oligodendrocyte functioning in pathogenesisis of white
    matter injury resulting from neonatal asphyxia” (2020-2021), NCN project no UMO-2020/36/T/N24/00144 (ETIUDA 8),  Principal investigator-Justyna Janowska
  •  “Analysis of interaction between mesenchymal stem cells and glioblastoma multiforme cancer stem cells” (2020 – 2021), NCN project no  2020/04/X/NZ3/00008 (MINIATURA 4), Principal investigator- Anna Andrzejewska
  • “Hypoxia-induced factors in altered oligodendrocyte differentiation in the in vitro model of neonatal hypoxia-ischemia” (2019 – 2021), NCN project no 1494/NZ3/N/31/2018 (PRELUDIUM 16), Principal investigator-Justyna Janowska,
  • ”Histone deacetylase inhibitors as potential modulatory factors of inflammatory response induced in the rat brain after neonatal asphyxia” (2018 – 2021), NCN project no 00582/NZ3/B/27/2017 (OPUS 17), Principal investigator-Malgorzata Nalecz,
  • ”Overexpression of neuregulin-1 in glial restricted progenitors (GRPs) – an approach to increase the functional properties of GRPs”(2018 – 2021), NCN project no 00721/NZ3/D/26/2017 (SONATA 13), Principal investigator- Luiza Stanaszek
  • “Exploitation of Regenerative Potential of Mesenchymal Stem Cells” (2016–2021), NCBiR project no STRATEGMEDI/235773/19/NCBR/2016,  Principal investigator- Miroslaw Janowski
  • „MRI-guided, intrathecal delivery of hydrogel-embedded glial progenitors for treatment of amyotrophic lateral sclerosis” (2016 – 2020) , NCBiR projectno 13/EuroNanoMed/2016, Principal investigator- Miroslaw Janowski

Collaboration

Domestic

  • Department of Orthopedicts and Traumatology, 1stt Faculty of Medicine,  Medical University of Warsaw, Warsaw
  • Warsaw University of Technology, Institute of Micromechanics and Photonics Engineering Division, Warsaw
  • University of Warsaw, Faculty of Biology, Warsaw
  • University of Warsaw, Centre of New Technologies, Warsaw
  • Centre for Specialized Surgery „Ortopedika”, Warsaw

Foreign

  • Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Verona, Italy
  • Département des Neurosciences Fondamentales, Université de Lausanne, Switzerland
  • Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland, Baltimore, Baltimore, MD, USA
  • R&D Department, FMC Biopolymer AS, Sandvika, Norway
  • NeuroDiderot, Université de Paris, Inserm, Paris, France
  • Department of Women's and Children's Health, University of Padova, Padova, Italy
  • Department of Molecular and Comparative Pathobiology and Neurology,  The Johns Hopkins University, School of Medicine, Baltimore, MD, USA
  • Perinatal Center, Institute of Neuroscience and Physiology,  Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
  • Life Sciences Department, University of Warwick, Coventry, UK
  • 3B’s Group, University of Minho, Braga, Portugal

Research equipment

  • Gel doc system (G-Box Chemi XR5 Syngen)
  • Termocycler vapo-protect (Eppendorf)
  • StepOne Real time PCR System (Applied Biosystems)
  • Spektrophotometer NanoDrop One (Termo-Scientific)
  • Shandon Cytospin 4 (Thermo electron Corporation)
  • Centrifuge (Eppendorf 5430R)
  • Electroporator Bio-RAD CE Module + Gene Pulser Xcell
  • Biological Safety Cabinets (MSC-Advantage Termo-Scientifix)
  • Incubator CO2-O2 Incubator NU-5830E
  • Incubator CO2-O2 Incubator NU-5831E
  • Freezer - ultra-low temperature (Eppendorf U410)
  • Apparatus for anesthesia(Matrx™ VIP 3000 Calibrated Vaporizer)
  • Stereotaxic Instrument
  • Surgical Microscope (Carl Zeiss OPMI pico)
  • Gas scavenger (Stoelting)
  • Surgical Microscope (Carl Zeiss OPMI pico)

Research methods

  • in vitro primarycultures of human mesenchymal stem cells and animal glial cells
  • Ex vivo cultures of organotypic hippocampal and brain slices
  • in vivo studies on animal models, including cytotoxic brain injury  (ouabain model), perinatal asphyxia, ALS (rag2/shi mice )
  • intraparenchymal and systemic cell transplantation in animal models of neurological diseases
  • microvesicles isolation from hBM-MSCs
  • functionality assessment of cell adhesion using microfluidic devices
  • cell tracing with different dyes (SPIO, GFP, PKH26, CMFDA)
  • immunocytochemistry and immunohistochemistry
  • advanced biochemical and molecular analyses, (e.g. electrophoresis, in situ zymography, Western Blotting, Sandwich ELISA tests, Luminex, cell transfection and GFP protein fusion, RT PCR, qRT PCR, methylation and acethylation of chromatine, etc)

Selected publications

  • Dabrowska S, Andrzejewska A, Kozlowska H, Strzemecki D, Janowski M, Lukomska B. Neuroinflammation evoked by brain injury in a rat model of lacunar infarct. Exp Neurol. 2021 Feb;336:113531. doi: 10.1016/j.expneurol.2020.113531
  • Janowska J, Gargas J, Ziemka-Nalecz M, Zalewska T, Sypecka J. Oligodendrocyte Response to Pathophysiological Conditions Triggered by Episode of Perinatal Hypoxia-Ischemia: Role of IGF-1 Secretion by Glial Cells. Mol Neurobiol. 2020;57(10):4250-4268 DOI:10.1007/s12035-020-02015-z.
  • Drela K, Stanaszek L, Snioch K, Kuczynska Z, Wrobel M, Sarzynska S, Legosz P, Maldyk P, Lukomska B. Bone marrow-derived from the human femoral shaft as a new source of mesenchymal stem/stromal cells: an alternative cell material for banking and clinical transplantation. Stem Cell Res Ther. 2020;11(1):262. DOI:10.1186/s13287-020-01697-5.
  • Zalewska T, Jaworska J, Sypecka J, Ziemka-Nalecz M.  Impact of a Histone Deacetylase Inhibitor-Trichostatin A on Neurogenesis after Hypoxia-Ischemia in Immature Rats. Int J Mol Sci. 2020;21(11):3808. DOI:10.3390/ijms21113808.
  • Andrzejewska A, Dabrowska S, Nowak B, Walczak P, Lukomska B, Janowski M.  Mesenchymal stem cells injected into carotid artery to target focal brain injury home to perivascular space. Theranostics. 2020;10(15):6615-6628. DOI: 10.7150/thno.43169.
  • Dabrowska S, Andrzejewska A, Strzemecki D, Muraca M, Janowski M, Lukomska B. Human bone marrow mesenchymal stem cell-derived extracellular vesicles attenuate neuroinflammation evoked by focal brain injury in rats. J Neuroinflammation. 2019 ;16(1):216. DOI:10.1186/s12974-019-1602-5.
  • Andrzejewska A, Nowakowski A,Grygorowicz T, Dabrowska S, Orzel J, Walczak P, Lukomska B, Janowski M. Single-cell, high-throughput, real-time in vitro analysis of mRNA-ITGA4-engineered mesenchymal stem cell docking to inflamed endothelium in a microfluidic model with validation in animals with focal brain injury. JCBF&M. 2019; 39:11:2308-2320. DOI:10.1177/0271678X18805238.
  • Andrzejewska A, Jablonska A, Podobinska M, Dabrowska S, Janowski M, Walczak P, Lukomska B. Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity. Stem Cell Res Ther. 2019;10(1):187. DOI:10.1186/s13287-019-1296-8.
  • Dabrowska S, Andrzejewska A, Lukomska B, Janowski M. Neuroinflammation as a target for treatment of stroke using mesenchymal stem cells and extracellular vesicles. J Neuroinflammation.2019;16(1):178. DOI:10.1186/s12974-019-1571-8.
  • Drela K, Nowakowski A, Stanaszek L, Kuczynska Z, Lukomska B. Experimental strategies of mesenchymal stem cells propagation - adverse events and potential risk of functional changes. Stem Cells Int. 2019;7012692. DOI:10.1155/2019/7012692.
  • Lukomska B, Stanaszek L, Zuba-Surma EK, Legosz P, Sarzynska S, Drela K.  Challenges and Controversies in Human Mesenchymal Stem Cells Therapy. Stem Cells Int. 2019;9628536.
  • Andrzejewska A, Lukomska B, Janowski M. Mesenchymal stem cells: from roots to boost. Stem Cells. 2019; 37(7):855-864. DOI:10.1186/s13287-019-1296-8.
  • Majchrzak M, Drela K, Andrzejewska A, Figurska S, Rogujski P, Fiedorowicz M, Walczak P, Janowski M, Lukomska B, Stanaszek L. A variable hSOD1 copy number influences the phenotypic outcome of SOD1/rag2 mice - immunocompetent model of ALS. Sci Rep. 2019;9(1):799. DOI:10.1038/s41598-018-37235-w.
  • Pawlak E, Janowski M, Habich A, Jablonska A, Sypecka J, Lukomska B. Intra-arterial infusion of human umbilical cord blood derived cells inversed learning asymmetry resulting from focal brain injury in rat. Front Neurol. 2019;10:786. DOI: 10.3389/fneur.2019.00786.
  • Lesniak WG, Chu C, Jablonska A, Du Y, Pomper MG, Walczak P, Janowski M. A Distinct Advantage to Intraarterial Delivery of 89Zr-Bevacizumab in PET Imaging of Mice With and Without Osmotic Opening of the Blood-Brain Barrier. J Nucl Med. 2019 ;60(5):617-622. DOI:10.2967/jnumed.118.218792.
  • Brzoska E, Kalkowski L, Kowalski K, Michalski P, Kowalczyk P, Mierzejewski B, Walczak P, Ciemerych MA, Janowski M.Muscular Contribution to Adolescent Idiopathic Scoliosis from the Perspective of Stem Cell-Based Regenerative Medicine. Stem Cells Dev. 2019;28(16):1059-1077. DOI:10.1089/scd.2019.0073.
  • Kasprzycka P, Archacka K, Kowalski K, Mierzejewski B, Zimowska M, Grabowska M, Piotrowski M, Rafalko M, Ryzko A, Irhashava A, Senderowski K, Golabek M, Streminska W, Janczyk-Ilach K, Koblowska M, Iwanicka-Nowicka R, Fogtman A, Janowski M, Walczak P, Ciemerych A, Brzoska E. The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts. Stem Cell Res Ther. 2019;10(1):343. DOI:10.1186/s13287-019-1444-1.
  • Golubczyk D, Malysz-Cymborska I, Kalkowski L, Janowski M, Coates JR, Wojtkiewicz J, Maksymowicz W, Walczak P. The Role of Glia in Canine Degenerative Myelopathy: Relevance to Human Amyotrophic Lateral Sclerosis. Mol Neurobiol. 2019; 56(8):5740-5748. DOI:10.1007/s12035-019-1488-3.
  • Janowska J, Gargas J, Ziemka-Nalecz M, Zalewska T, Buzanska L, Sypecka J. Directed glial differentiation and transdifferentiation for neural tissue regeneration. Exp Neurol. 2019; 319:112813. DOI:10.1016/j.expneurol.2018.08.010.
  • Jaworska J, Zalewska Z, Sypecka J, Ziemka-Nalecz M. Effect of HDAC inhibitor, Sodium butyrate, on neurogenesis in a rat model of neonatal hypoxia-ischemia. Potential mechanism of action. Mol Neurobiol. 2019;56(9):6341-6370. DOI:10.1007/s12035-019-1518-1.
  • Théry C, Witwer KW, Lukomska B et al. Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. 2018:8:1, 1535750.
  • Dabrowska S, Sypecka J, Jablonska, Strojek L, Wielgos M, Domanska-Janik K, Sarnowska A. Neuroprotective Potential and Paracrine Activity of Stromal Vs. Culture-Expanded hMSC Derived from Wharton Jelly under Co-Cultured with Hippocampal Organotypic Slices. Mol Neurobiol. 2018;55(7):6021-6036.                       
  • Dabrowska  S, Del Fattore A, Karnas E, Frontczak-Baniewicz, Kozlowska H, Muraca M, Janowski M, Lukomska B. Imaging of extracellular vesicles derived from human bone marrow mesenchymal stromal cells using fluorescent and magnetic labels. Int J Nanomedicine. 2018;13:1653-1664.
  • Oliveira JM, Carvalho L,  Silva-Correia J, Vieira S,  Majchrzak M, Lukomska B, Stanaszek L, Strymecka K, Malysz-Cymborska I, Golubczyk D, Kalkowski L, Reis RL, Janowski M, Walczak P. Hydrogel-based scaffolds to support intrathecal stem cell transplantation as a gateway to the spinal cord: clinical needs, biomaterials and imaging technologies. NPJ Regen Med. 2018;3:8.
  • Guzman R, Janowski M, Walczak P. Intra-arterial delivery of cell therapies for stroke. Stroke. 2018;49:1075-1082.
  • Srivastava RK, Bulte JWM, Walczak P, Janowski M. Migratory potential of transplanted glial progenitors as critical factor for successful translation of glia replacement therapy: the gap between mice and men. Glia. 2018;66(5):907-919.
  • Janowska J, Sypecka J. Therapeutic Strategies for Leukodystrophic Disorders Resulting from Perinatal Asphyxia: Focus on Myelinating Oligodendrocytes. Mol Neurobiol. 2018;55(5):4388-4402.
  • Janowska J, Gargas J, Ziemka-Nalecz M, Zalewska T,Buzanska L, Sypecka J. Directed glial differentiation and transdifferentiation for neural tissue regeneration. Exp Neurol. 2018.DOI:S0014-4886(18)30401-1.
  • Ziemka-Nalecz M, Jaworska J, Sypecka J, Zalewska T. Histone deacetylase inhibitors: a therapeutic key in neurological disorders? J Neuropathol Exp Neurol. 2018;77(10):855-870.
  • Ziemka-Nalecz M, Janowska J, Strojek L, Jaworska J, Zalewska T, Frontczak-Baniewicz M, Sypecka J. Impact of neonatal hypoxia-ischemia on oligodendrocyte survival, maturation and myelinating potential. J Cellular Mol Med. 2018;22(1):207-222.
  • Janowska J, Ziemka-Nalecz M, Sypecka J. Differentiation of rat oligodendroglial cells is highly influenced by oxygen tension: in vitro model mimicking physiologically normoxic conditions. Int J Mol Sci. 2018;24:19(2):331.
  • Nowakowski A,  Andrzejewska A, Boltze J, Nitzsche F, Cui L, Jolkkonen J, Walczak P, Lukomska B,  Janowski M.  Translation, but not transfection limits clinically relevant, exogenous mRNA based induction of alpha-4 integrin expression on human mesenchymal stem cells. Sci Rep. 2017:7(1):1103.
  • Nitzsche F, Müller C, Lukomska B, Jolkkonen J, Deten A, Boltze J. The MSC adhesion cascade - insights into homing and transendothelial migration. Stem Cells. 2017; 35(6):1446-1460.
  • Walczak P, Wojtkiewicz J, Nowakowski A, Chehade M, Habich A, Holak P, Xu J, Adamiak Z, Pearl M,  Gailloud P, Lukomska B, Maksymowicz W, Bulte JWM, Janowski M. Real-time MRI for precise and predictable intra-arterial stem cell delivery to the central nervous system. J Cereb Blood Flow Metab. 2017;37(7):2346-2358.
  • Ziemka-Nalecz M, Jaworska J, Zalewska T. Insights into the neuroinflammatory responces after neonatal hypoxia-ischemia J Neuropath Exp Neurology. 2017; 76(8):644-654.
  • Ziemka-Nalecz M, Jaworska J, Sypecka J, Polowy R, Filipkowski R, Zalewska T. Sodium butyrate, a histone deacetylase inhibitor, exhibits  neuroprotective/neurogenic effects in a rat model of neonatal hypoxia –ischemia. Mol  Neurobiol. 2017;54(7):5300-5318.
  • Jaworska J, Ziemka-Nalecz M, Sypecka J, Zalewska T. The potential neuroprotective role of histone deacetylase inhibitor Sodium Butyrate after neonatal hypoxia-ischemia. J Neuroinflammation. 2017;14(1):34.
  • Sypecka J, Ziemka-Nalecz M, Dragun -Szymczak P, Zalewska T. A simple, xeno-free method for oligodendrocyte derivation from human neural stem cells derived from umbilical cord: engagement of gelatinases in the cell commitment and differentiation. J Tissue Eng Regen Med. 2017;11(5):1442-1455.