Imaging of extracellular vesicles derived from human bone marrow mesenchymal stem cells using fluorescent and magnetic labels

Int J Nanomedicine. 2018 Mar 19;13:1653-1664. doi: 10.2147/IJN.S159404. eCollection 2018.

Dabrowska S1, Del Fattore A2, Karnas E3,4, Frontczak-Baniewicz M5, Kozlowska H6, Muraca M7, Janowski M1,8, Lukomska B1.

1 NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.

2 Multifactorial Disease and Complex Phenotype Research Area, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

3 Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.

4 Malopolska Centre of Biotechnology, Krakow, Poland.

5 Electron Microscopy Platform, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.

6 Laboratory of Advanced Microscopy Techniques, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.

7 Department of Women's and Children's Health, University of Padua, Padua, Italy.

8 Russel H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Imaging of extracellular vesicles derived from human bone marrow mesenchymal stem cells using fluorescent and magnetic labels.

 

ABSTRACT

Mesenchymal stem cells have been shown therapeutic in various neurological disorders. Recent studies support the notion that the predominant mechanism by which MSCs act is through the release of extracellular vesicles (EVs). EVs seem to have similar therapeutic activity as their cellular counterparts and may represent an interesting alternative standalone therapy for various diseases. The aim of the study was to optimize the method of EV imaging to better understand therapeutic effects mediated by EVs.

 

KEYWORDS:

MRI; cell tracking; extracellular vesicles; fluorescent dye; iron oxide; mesenchymal stem cells

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