Department of Human Epigenetics

Head
Monika Puzianowska-Kuźnicka, MD, PhD, phone +48 22 6086591, This email address is being protected from spambots. You need JavaScript enabled to view it.
Secretariat
Agnieszka Włodarczyk, phone/fax. +48 22 6086410, This email address is being protected from spambots. You need JavaScript enabled to view it.
Research staff
Edward Franek, MD, PhD, phone +48 22 5081405, This email address is being protected from spambots. You need JavaScript enabled to view it.
Alina Kuryłowicz, MD, PhD, phone +48 22 6086406,This email address is being protected from spambots. You need JavaScript enabled to view it.
Monika Slupecka-Ziemilska, PhD, phone 22 6086401, This email address is being protected from spambots. You need JavaScript enabled to view it.
Magdalena Walicka, MD, PhD, phone +48 22 5081405, This email address is being protected from spambots. You need JavaScript enabled to view it.
Marta Cąkała-Jakimowicz, PhD, phone +48 22 6086406,This email address is being protected from spambots. You need JavaScript enabled to view it.
Anna Domaszewska-Szostek, PhD, phone +48 22 6086404,This email address is being protected from spambots. You need JavaScript enabled to view it.
Marta Jonas, MD, PhD, phone +48 22 6086406,This email address is being protected from spambots. You need JavaScript enabled to view it.
Technical staff
Joanna Borkowska, PhD, phone +48 22 6086404,This email address is being protected from spambots. You need JavaScript enabled to view it.
med. Arkadiusz Krysiński, 22 5081405, This email address is being protected from spambots. You need JavaScript enabled to view it.
Olga Bujanowska, MSc, phone +48 22 6086410,This email address is being protected from spambots. You need JavaScript enabled to view it.
Małgorzata Szwed, phone +48 22 60 6406,This email address is being protected from spambots. You need JavaScript enabled to view it.
Barbara Buczek, phone +48 22 6086410,This email address is being protected from spambots. You need JavaScript enabled to view it.

PhD students
Martyna Nowicka, MSc

Graduate students
Eryk Chaciński, Warsaw University of Life Sciences
Interns
Jakub Podraza, Warsaw Unversity of Life Sciences

Research profile

  • Phenotypes of aging. Extreme longevity.
  • Genetics and epigenetics of extreme longevity.
  • Epigenetics of skin aging.
  • Genetics and epigenetics of obesity.
  • Age-related diseases: obesity, type 2 diabetes, osteoporosis. Type 1 diabetes.
  • The effect of epigenetic programming on health later in life.

 

Grants

present

  • The effect of maternal treatment with metformin in pregnancy and lactation on programming metabolic adverse conditions in the offspring – long-term functional and epigenetic studies, the National Science Centre SONATA BIS grant no 2022/46/E/NZ7/00286, leader: Monika Slupecka-Ziemilska, PhD, 2023-07.2027
  • The role of senescent cells in the human epidermis and the study of the influence of selected flavonoids on the elimination of their secretory phenotype, the MMRI Research Fund grant no.FBW 005/2021, leader: Anna Domaszewska-Szostek, PhD, 11.2021-10.2023.

completed

  • Selected polyphenols as potential senolytic drugs modulating the secretory phenotype associated with senescence (SASP) in human keratinocytes, the National Science Centre MINIATURA grant no. 2020/04/X/NZ4/02074, leader: Anna Domaszewska-Szostek, PhD, 11.2021-11.2022.
  • Epigenetic regulation of estrogen metabolism in adipose tissue of obese individuals, the National Science Centre OPUS grant no. 2018/31/B/NZ5/01556, leader: Alina Kuryłowicz, MD, PhD, 07.2019-06.2023.
  • The role of long non-coding RNA (lncRNA) in the pathogenesis of adipose tissue dysfunction in human obesity, the MMRI Research Fund grant no. FBW 007/2021, leader: Alina Kuryłowicz, MD, PhD, 2021-04.2022.
  • Effect of metformin treatment in pregnancy and lactation on programming the aging process in offspring - the role of sirtuin 1, the MMRI Research Fund grant no. FBW 003/2020, leader: Monika Słupecka-Ziemilska, PhD, 10.2020-12.2021.
  • Differences in the concentrations of pro- and anti-inflammatory cytokines in the blood of healthy long-lived people maintaining physical and intellectual fitness, and those who are disabled or suffer from age-related diseases, the MMRI Research Fund grant no. FBW009/2020, leader: Marta Jonas, MD, PhD, 10.2020-12.2021.
  • A randomized, open-label, two-arm clinical trial comparing the effectiveness of: 1. azithromycin + standard of care treatment regimen with standard of care treatment regimen in hospitalized patients diagnosed with mild SARS-CoV-2 infection (MEWS 0-2 points) and 2. azithromycin + standard of care treatment regimen with azithromycin + tocilizumab + standard of care treatment regimen in hospitalized patients diagnosed with mild/severe to severe SARS-CoV-2 infection (MEWS ≥ 3 points), the Medical Research Agency grant no. 2020/ABM/COVID19/0009, leader: Edward Franek, MD, PhD, 05.2020-11.2021.
  • Participation of TET enzymes in the aging of human skin, the National Science Centre OPUS grant no. 2014/13/B/NZ4/00157, leader: Monika Puzianowska-Kuźnicka, MD, PhD, 01.2015-11.2018.
  • The role of the gonadotropin-releasing hormone receptor pathway in B-cell function, the National Science Centre PRELUDIUM grant no. 2014/15/N/NZ3/04483, leader: Dorota Walkiewicz, MSc, 08.2015-08.2018.
  • The potential impact of epigenetic drift on age-dependent change in SIRT6 expression in human blood mononuclear cells, the National Science Centre PRELUDIUM grant no. 2013/09/N/NZ3/01363, leader: Magdalena Owczarz, MSc, 03.2014-03.2017
  • Obesity in humans: the potential role of sirtuin genes and epigenetic control of their activity, the National Science Centre OPUS grant no. 2012/05/B/NZ5/01536, leader: Alina Kuryłowicz, MD, PhD, 02.2013-08.2016.
  • Polish reference genome for genomic diagnostics and personalized medicine, the National Centre for Research and Development grant no 181852, contract no. INNOTECH-K2/IN2/10/181852/NCBR/13, subproject leader: Monika Puzianowska-Kuźnicka, MD, PhD, 01.2013-02.2016.
  • Study of the effect of vitamin D levels in adipose tissue and serum on inflammation in obese subjects, the Scientific Research Committee/National Science Center grant no. N N402 467939, leader: Marta Jonas, MD, PhD, 09.2010-03.2014.
  • Aging of the immune system: studying the expression of genes influencing the rate of the aging process and the mechanisms of their epigenetic regulation in blood mononuclear cells of young, elderly and long-lived people, the Ministry of Science and Higher Education grant no. N N401 037338, leader: Monika Puzianowska-Kuźnicka, MD, PhD, 03.2010-06.2013.

Collaboration
domestic

  • International Institute of Molecular and Cell Biology, Warsaw, Poland.
  • Department of Geriatrics and Gerontology, Department of Internal Medicine and Gerontocardiology, Department of General, Oncological and Bariatric Surgery, Medical Centre of Postgraduate Education, Warsaw, Poland.
  • Department of Internal Medicine, Endocrinology, and Diabetology, MSWiA Central Hospital, Warsaw, Poland.
  • The Kielanowski Institute of Animal Physiology and Nutrition, PAS, Jabłonna, Poland.

foreign

  • Department of Anesthesiology, University of Pennsylvania, Philadelphia, PA, USA.
  • Institut für Mikrobiologie und Hygiene, Charité – Universittätsmedizin Berlin, Berlin, Germany.
  • Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.

Research equipment

  • Equipment for molecular biology, biochemistry, and cell culture.

Selected publications

  • Gutowska K, Czajkowski K, Kuryłowicz A. Receptor for the advanced glycation end products (RAGE) pathway in adipose tissue metabolism. Int J Mol Sci. 2023;24:10982. DOI: 10.3390/ijms241310982.
  • Kuryłowicz A. Estrogens in adipose tissue physiology and obesity-related dysfunction. Biomedicines. 2023;11:690. DOI: 10.3390/biomedicines11030690. 
  • Kołodziej-Wojnar P, Borkowska J, Domaszewska-Szostek A, Bujanowska O, Noszczyk B, Krześniak N, Stańczyk M, Puzianowska-Kuznicka M. Ten-eleven translocation 1 and 2 enzymes affect human skin fibroblasts in an age-related manner. Biomedicines. 2023;11:1659. DOI: 10.3390/biomedicines11061659.
  • Domaszewska-Szostek A, Polak A, Słupecka-Ziemilska M, Krzyżanowska M, Puzianowska-Kuznicka M. Current status of cel-based therapies for vitiligo. Int J Mol Sci. 2023;24:3357. DOI: 10.3390/ijms24043357.
  • Starzyńska A, Wychowański P, Nowak M, Sobocki BK, Jereczek-Fossa BA, Słupecka-Ziemilska M. Association between maternal periodontitis and development of systematic diseases in offspring. Int J Mol Sci. 2022;23:2473. DOI: 10.3390/ijms23052473.
  • Hall M, Walicka M, Panczyk M, Traczyk I. Assessing long-term impact of dietary interventions on occurrence of symptoms consistent with hypoglycemia in patients without diabetes: a one-year follow-up study. Nutrients. 2022;14:497. DOI: 10.3390/nu14030497
  • Franek E, Gerstein HC, Riddle MC, Nicolay C, Hickey A, Botros FT, Loo LS. Efficacy and safety outcomes of dulaglutide by baseline HbA1c: a post hoc analysis of the REWIND trial. Diabetes Obes Metab. 2022;24:1753-1761. DOI: 10.1111/dom.14760.
  • Russo C, Walicka M, Caponnetto P, Cibella F, Maglia M, Alamo A, Campagna D, Frittitta L, Di Mauro M, Caci G, Krysinski A, Franek E, Polosa R. Efficacy and safety of varenicline for smoking cessation in patients with type 2 diabetes: a randomized clinical trial. JAMA Netw Open. 2022;5:e2217709. DOI: 10.1001/jamanetworkopen.2022.17709.
  • Gerstein HC, Ramasundarahettige C, Avezum A, Basile J, Conget I, Cushman WC, Dagenais GR, Franek E, Lakshmanan M, Lanas F, Leiter LA, Pogosova N, Probstfield J, Raubenheimer PJ, Riddle M, Shaw J, Sheu WH, Temelkova-Kurktschiev T, Turfanda I, Xavier D. A novel kidney disease index reflecting both the albumin-to-creatinine ratio and estimated glomerular filtration rate, predicted cardiovascular and kidney outcomes in type 2 diabetes. Cardiovasc Diabetol. 2022;21:158. DOI: 10.1186/s12933-022-01594-6.
  • Słupecka-Ziemilska M, Pierzynowski SG, Szczurek P, Pierzynowska K, Wychowański P, Seklecka B, Koperski M, Starzyńska A, Szkopek D, Donaldson J, Andrzejewski K, Woliński J. Milk formula enriched with sodium butyrate influences small intestine contractility in neonatal pigs. Nutrients. 2022;14:4301. DOI: 10.3390/nu14204301.
  • Zaworski K, Woliński J, Słupecka-Ziemilska M, Pierzynowski S, Pierzynowska K. Pre-digestion of the lipids in infant formula affects gut maturation of the preterm pig. PLoS One. 2022;17:e0265144. DOI: 10.1371/journal.pone.0265144.
  • Cukierman-Yaffe T, Gerstein HC, Basile J, Bethel MA, Cardona-Muñoz EG, Conget I, Dagenais G, Franek E, Hall S, Hancu N, Jansky P, Lakshmanan M, Lanas F, Leiter LA, Lopez-Jaramillo P, Pirags V, Pogosova N, Probstfield J, Rao-Melacini P, Ramasundarahettige C, Raubenheimer PJ, Riddle MC, Rydén L, Shaw JE, Sheu WH, Temelkova-Kurktschiev T. Novel indices of cognitive impairment and incident cardiovascular outcomes in the REWIND trial. J Clin Endocrinol Metab. 2022;107:e3448-e3454. DOI: 10.1210/clinem/dgac200.
  • Puzianowska-Kuznicka M, Kurylowicz A, Wierucki L, Owczarek AJ, Jagiello K, Mossakowska M, Zdrojewski T, Chudek J. Obesity in Caucasian seniors on the rise: Is it truly harmful? Results of the PolSenior 2 study. Nutrients. 2022;14:4621. DOI: 10.3390/nu14214621.
  • Hadar A, Voinsky I, Parkhomenko O, Puzianowska-Kuźnicka M, Kuźnicki J, Gozes I, Gurwitz D. Higher ATM expression in lymphoblastoid cell lines from centenarian compared with younger women. Drug Dev Res. 2022;83:1419-1424. DOI: 10.1002/ddr.21972.
  • Koźniewski K, Wąsowski M, Jonas MI, Lisik W, Jonas M, Binda A, Jaworski P, Tarnowski W, Noszczyk B, Puzianowska-Kuznicka M, Kuryłowicz A. Epigenetic regulation of estrogen receptor genes’ expression in adipose tissue in the course of obesity. Int J Mol Sci. 2022;23:5989. DOI: https://doi.org/10.3390/ijms23115989 
  • Cąkała-Jakimowicz M, Puzianowska-Kuznicka M. Towards understanding the lymph node respose to skin infection with saprophytic Staphylococcus epidermidis. Biomedicines. 2022;10:1021. DOI:10.3390/biomedicines10051021.
  • Biernacka-Bartnik A, Kocełak P, Owczarek AJ, Choręza P, Puzianowska-Kuźnicka M, Markuszewski L, Madej P, Chudek J, Olszanecka-Glinianowicz M. Prediction of insulin resistance and impaired fasting glucose based on sex hormone-binding globulin (SHBG) levels in polycystic ovary syndrome. Int J Endocrinol. 2022;2022:6498768. DOI: 10.1155/2022/6498768. 
  • Yang W, Akhtar S, Franek E, Haluzík M, Hirose T, Kalyanam B, Kar S, Wu T, Gogas Yavuz D, Unnikrishnan AG. Postprandial glucose excursions in Asian versus non-Asian patients with type 2 diabetes: A post hoc analysis of baseline data from phase 3 randomised controlled trials of IDegAsp. Diabetes Ther. 2022;13:311-323. DOI: 10.1007/s13300-021-01196-7.
  • Domaszewska-Szostek A, Gewartowska M, Stanczyk M, Narowska B, Moscicka-Wesolowska M, Olszewski W. An anhydrous sodium chloride skin preservation model for studies on keratinocytes grafting into the wounds. Pharmaceutics. 2021;13:2078. DOI: 10.3390/pharmaceutics13122078.
  • Domaszewska-Szostek A, Krzyżanowska MO, Czarnecka AM, Siemionow M. Local treatment of burns with cell-based therapies tested in clinical studies. J Clin Med. 2021;10:396. DOI: 10.3390/jcm10030396. 
  • Styszyński A, Chudek J, Mossakowska M, Lewandowski K, Puzianowska-Kuźnicka M, Klich-Rączka A, Więcek A, Wieczorowska-Tobis K. Causes of anemia in Polish older population – Results from the PolSenior study. Cells. 2021;10:2167. DOI: 10.3390/cells10082167. 
  • Walkiewicz D, Wicik Z, Puzianowska-Kuznicka M. Gonadotropin-releasing hormone receptor pathway affects the function of human EBV-transformed B lymphocytes in an age-independent way. Exp Gerontol. 2021;152:111471. DOI: 10.1016/j.exger.2021.111471.
  • Lingvay I, Buse JB, Franek E, Hansen MV, Koefoed MM, Mathieu C, Pettus J, Stachlewska K, Rosenstock J. A randomized, open-label comparison of once-weekly insulin icodec titration strategies versus once-daily insulin glargine U100. Diabetes Care. 2021;44:1595-1603. DOI: 10.2337/dc20-2878.
  • Bajaj HS, Gerstein HC, Rao-Melacini P, Basile J, Colhoun H, Conget I, Cushman WC, Dagenais GR, Franek E, Hanefeld M, Keltai M, Lakshmanan M, Lanas F, Leiter LA, Lopez-Jaramillo P, Pirags V, Pogosova N, Probstfield J, Raubenheimer P, Ryden L, Shaw JE, Sheu WH, Xavier D. Erectile function in men with type 2 diabetes treated with dulaglutide: an exploratory analysis of the REWIND placebo-controlled randomised trial.  Lancet Diabetes Endocrinol. 2021;9:484-490. DOI: 10.1016/S2213-8587(21)00115-7.
  • Broadwell A, Chines A, Ebeling PR, Franek E, Huang S, Smith S, Kendler D, Messina O, Miller PD. Denosumab safety and efficacy among subjects in the FREEDOM extension study with mild-to-moderate chronic kidney disease. J Clin Endocrinol Metab. 2021;106:397-409. DOI: 10.1210/clinem/dgaa851.
  • Riddle MC, Gerstein HC, Xavier D, Cushman WC, Leiter LA, Raubenheimer PJ, Atisso CM, Raha S, Varnado OJ, Konig M, Lakshmanan M, Franek E. Efficacy and safety of dulaglutide in older patients: A post-hoc analysis of the REWIND trial. J Clin Endocrinol Metab. 2021;106:1345-1351. DOI: 10.1210/clinem/dgab065.
  • Cakala-Jakimowicz M, Kolodziej-Wojnar P, Puzianowska Kuznicka M. Aging-related cellular, structural and functional changes in the lymph nodes: A significant component of immunosenescence? An overview. Cells. 2021;10:3148. DOI: 10.3390/cells10113148.
  • Kuryłowicz A. microRNAs in human adipose tissue physiology and dysfunction. Cells. 2021;10:3342. DOI:  https://doi.org/10.3390/cells10123342.
  • Domaszewska-Szostek A, Puzianowska-Kuźnicka M, Kuryłowicz A. Flavonoids in skin senescence prevention and treatment. Int J Mol Sci. 2021;22:6814. DOI: 10.3390/ijms22136814
  • Walicka M, Tuszyńska A, Chlebus M, Sanchak Y, Śliwczyński A, Brzozowska M, Rutkowski D, Puzianowska-Kuźnicka M, Franek E. Predictors of in-hospital mortality in surgical wards: A multivariable retrospective cohort analysis of 2,800,069 hospitalizations. World J Surg.2021;45:480- DOI: 10.1007/s00268-020-05841-3.
  • Walicka M, Chlebus M, Śliwczyński A, Brzozowska M, Rutkowski D, Czech M, Tuszyńska A, Jacyna A, Puzianowska-Kuźnicka M, Franek E. Predictors of in-hospital mortality in nonsurgical departments: a multivariable regression analysis of 2 855 029 hospitalizations. Pol Arch Intern Med. 2020;130:268-275. DOI: 10.20452/pamw.15185.
  • Słupecka-Ziemilska M, Szczurek P, Boryczka M, Gajewska M, Wychowański P, Kuwahara A, Kato I, Dzięgelewska Z, Woliński J. The effects of intra-stomach obestatin administration on intestinal contractility in neonatal piglets fed milk formula PLoS One. 2020;15:e0230190. DOI:10.1371/journal.pone.0230190.
  • Cukierman-Yaffe T, Gerstein HC, Colhoun HM, Diaz R, García-Pérez LE, Lakshmanan M, Bethel A, Xavier D, Probstfield J, Riddle MC, Rydén L, Atisso CM,  Hall S, Rao-Melacini P, Basile J, Cushman WC, Franek E, Keltai M, Lanas F, Leiter LA, Lopez-Jaramillo P, Pirags V, Pogosova N, Raubenheimer PJ, Shaw JE, Sheu WHH, Temelkova-Kurktschiev Effect of dulaglutide on cognitive impairment in type 2 diabetes: an exploratory analysis of the REWIND trial. Lancet Neurol. 2020;19:582-590. DOI:10.1016/S1474-4422(20)30173-3.
  • Słupecka-Ziemilska M, Wychowański P, Puzianowska-Kuznicka M. Gestational diabetes mellitus affects offspring’s epigenome. Is there a way to reduce the negative consequences? Nutrients. 2020;2:2792. dOI:10.3390/nu12092792.
  • Kuryłowicz A, Puzianowska-Kuźnicka M. Induction of adipose tissue browning as a strategy to combat obesity. Int J Mol Sci. 2020;21:E6241. DOI: 10.3390/ijms21176241.
  • Owczarz M, Połosak J, Domaszewska-Szostek A, Kołodziej P, Kuryłowicz A, Puzianowska-Kuznicka M. Age-related epigenetic drift deregulates SIRT6expression and affects its downstream genes in human peripheral blood mononuclear cells. Epigenetics. 2020;15:1336-1347. doi: 10.1080/15592294.2020.1780081.
  • Kuryłowicz A, Koźniewski K. Anti-inflammatory strategies targeting metaflammation in type 2 diabetes. Molecules. 2020;25:E2224. DOI:10.3390/molecules25092224.
  • Lane WS, Favaro E, Rathor N, Jang HC, Kjærsgaard MIS, Oviedo A, Rose L, Senior P, Sesti G, Gonzalez AS, Franek E. A randomized trial evaluating the efficacy and safety of fast-acting insulin aspart compared with insulin aspart, both in combination with insulin degludec with or without metformin, in adults with type 2 diabetes (Onset 9). Diabetes Care. 2020;43:1710-1716. DOI:10.2337/dc19-2232.
  • Woliński J, Szczurek P, Pierzynowska K, Wychowański P, Seklecka B, Boryczka M,  Kuwahara A, Kato I, Drahanchuk O, Zaworski K, Pierzynowski SG, Słupecka-Ziemilska M. Influence of obestatin on the histological development of the small intestine in piglets during the first week of postnatal life. Animal. 2020;1‐9. DOI:10.1017/S1751731120000919 [Epub ahead of print].
  • Hamann L, Szwed M, Mossakowska M, Chudek J, Puzianowska-Kuznicka M. First evidence for STING SNP R293Q being protective regarding obesity-associated cardiovascular disease in age-advanced subjects - a cohort study. Immun Ageing. 2020;17:7. DOI:10.1186/s12979-020-00176-y.
  • Kuryłowicz A, Cakała-Jakimowicz M, Puzianowska-Kuźnicka M. Targeting abdominal obesity and its complications with dietary phytoestrogens. Nutrients. 2020;12:582. DOI: 10.3390/nu12020582.
  • Borkowska J, Domaszewska-Szostek A, Kołodziej P, Wicik Z, Połosak J, Buyanovskaya O, Charzewski L, Stańczyk M, Noszczyk B, Puzianowska-Kuznicka M. Alterations in 5hmC level and genomic distribution in aging-related epigenetic drift in human adipose stem. Epigenomics. 2020;12:423-437. DOI:10.2217/epi-2019-0131.
  • Puzianowska-Kuznicka M, Kuryłowicz A, Walkiewicz D, Borkowska J, Owczarz M, Olszanecka-Glinianowicz M, Wieczorowska-Tobis K, Skalska A, Szybalska A, Mossakowska M. Obesity paradox in Caucasian seniors: results of the PolSenior study. J Nutr Health Ageing. 2019;23:796-804. DOI: 10.1007/s12603-019-1257-z.
  • Jonas M, Kurylowicz A, Bartoszewicz Z,  Lisik W, Jonas M, Kozniewski K, Puzianowska-Kuznicka M. Vitamin D receptor gene expression in adipose tissue of obese individuals is regulated by miRNAs and correlates with pro-inflammatory cytokines level. Int J Mol Sci. 2019;20(21):5272. DOI: 10.3390/ijms20215272.
  • Gerstein HC, Colhoun HM, Dagenais GR, Diaz R, Lakshmanan M, Pais P, Probstfield J, Riesmeyer JS, Riddle MC, Rydén L, Xavier D, Atisso CM, Dyal L, Hall S, Rao-Melacini P, Wong G, Avezum A, Basile J, Chung N, Conget I, Cushman WC, Franek E, Hancu N, Hanefeld M, Holt S, Jansky P, Keltai M, Lanas F, Leiter LA, Lopez-Jaramillo P, Cardona Munoz EG, Pirags V, Pogosova N, Raubenheimer PJ, Shaw JE, Sheu WH, Temelkova-Kurktschiev T; REWIND Investigators. Dulaglutide and cardiovascular outcomes in type 2 diabetes (REWIND): a double-blind, randomized placebo-controlled trial. Lancet. 2019;394:121-130. DOI: 10.1016/S0140-6736(19)31149-3.
  • Gerstein HC, Colhoun HM, Dagenais GR, Diaz R, Lakshmanan M, Pais P, Probstfield J, Botros FT, Riddle MC, Rydén L, Xavier D, Atisso CM, Dyal L, Hall S, Rao-Melacini P, Wong G, Avezum A, Basile J, Chung N, Conget I, Cushman WC, Franek E, Hancu N, Hanefeld M, Holt S, Jansky P, Keltai M, Lanas F, Leiter LA, Lopez-Jaramillo P, Cardona Munoz EG, Pirags V, Pogosova N, Raubenheimer PJ, Shaw JE, Sheu WH, Temelkova-Kurktschiev T; REWIND Investigators. Dulaglutide and renal outcomes in type 2 diabetes: an exploratory analysis of the REWIND randomised, placebo-controlled trial. Lancet. 2019;394:131-138. DOI: 10.1016/S0140-6736(19)31150-X.
  • Pratley RE, Emerson SS, Franek E, Gilbert MP, Marso SP, McGuire DK, Pieber TR, Zinman B, Hansen CT, Hansen MV, Mark T, Moses AC, Buse JB; DEVOTE Study Group. Cardiovascular safety and lower severe hypoglycaemia of insulin degludec versus insulin glargine U100 in patients with type 2 diabetes aged 65 years or older: results from DEVOTE (DEVOTE 7). Diabetes Obes Metab. 2019;21:1625-1633. DOI: 10.1111/dom.13699.
  • Olczak E, Kuryłowicz A, Wicik Z, Kolodziej P, Cakala-Jakimowicz M, Buyanovskaya O, Slusarczyk P, Mossakowska M, Puzianowska-Kuznicka M. Glucocorticoid receptor (NR3C1) gene polymorphisms are associated with age and blood parameters in Polish Caucasian nonagenarians and centenarians. Exp Gerontol. 2019;116:20-24. DOI: 10.1016/j.exger.2018.12.006.
  • Walicka M, Jozwiak J, Rzeszotarski J, Zonenberg A, Masierek M, Bijos P, Franek E. Diagnostic accuracy of glycated haemoglobin and average glucose values in type 2 diabetes mellitus treated with premixed Insulin. Diabetes Ther. 2019;10:587-596. DOI: 10.1007/s13300-019-0570-1.
  • Hamann L, Ruiz-Moreno JS, Szwed M, Mossakowska M, Lundvall L, Schumann RR, Opitz B, Puzianowska-Kuznicka M. STING SNP R293Q is associated with a decreased risk of aging-related diseases. Gerontology. 2019;65:145-154. DOI: 10.1159/000492972.
  • Gilbert MP, Bain SC, Franek E, Jodar-Gimeno E, Naucck MA, Pratley R, Roginski R, Kerr Saraiva JF, Rasmussen S, Buse JB. Effect of liraglutide on cardiovascular outcomes in elderly patients: A post-hoc analysis of a randomized controlled trial. Ann Int Med. 2019;170:423-426. DOI: 10.7326/M18-1569.
  • Laudanski K, Zawadka M, Polosak J, Modi J, DiMeglio M, Gutsche J, Szeto WY, Puzianowska-Kuznicka M. Acquired immunological imbalance after surgery with cardiopulmonary bypass due to epigenetic over-activation of PU.1/M-CSF. J Transl Med. 2018;16:143. DOI: 10.1186/s12967-018-1518-3.
  • Hadar A, Milanesi E, Walczak M, Puzianowska-Kuznicka M, Kuźnicki J, Squassina A, Niola P, Chillotti C, Attems J, Gozes I, Gurwitz D. SIRT1, miR-132 and miR-212 link human longevity to Alzheimer’s Disease. Scientific Rep. 2018;8:8465. DOI: 10.1038/s41598-018-26547-6.
  • Sulicka J, Pac A, Puzianowska-Kuznicka M, Zdrojewski T, Chudek J, Tobiasz-Adamczyk B, Mossakowska M, Skalska A, Więcek A, Grodzicki T. Health status of older cancer survivors—results of the PolSenior study. J Cancer Surv. 2018;12:326-333. DOI: 10.1007/s11764-017-0672-6.
  • Mathieu C, Bode BW, Franek E, Philis-Tsimikas A, Rose L, Graungaard T, Birk Østerskov A, Russell-Jones D. Efficacy and safety of fast-acting insulin aspart in comparison with insulin aspart in type 1 diabetes (onset 1): a 52-week, randomized, treat-to-target, phase 3 trial. Diabetes Obes Metab. 2018;20:1148-1155. DOI: 1111/dom.13205. 
  • Ruiz-Moreno J, Hamann L, Shah JA, Verbon A, Mockenhaupt FP, Puzianowska-Kuznicka M, Naujoks J, Sander LE, Witzenrath M, Cambier JC, Suttorp N, Schumann RR, Jin L, Hawn TR, Opitz B, CAPNETZ Study Group. The common HAQ STING variant impairs cGAS-dependent antibacterial responses and is associated with susceptibility to Legionnaires' disease in humans. PLOS Pathogenes. 2018;14:e1006829. DOI: 10.1371/journal.ppat.1006829.
  • Kuryłowicz A, Wicik Z, Owczarz M, Jonas MI, Kotlarek M, Świerniak M, Lisik W, Jonas M, Noszczyk B, Puzianowska-Kuznicka M. NGS reveals molecular pathways affected by obesity and weight loss-related changes in miRNA levels in adipose tissue. Int J Mol Sci. 2017;19. pii: E66. DOI: 10.3390/ijms19010066.
  • Jonas MI, Kurylowicz A, Bartoszewicz Z, Lisik W, Jonas M, Domieniek-Karlowicz J, Puzianowska-Kuznicka M. Adiponectin/resistin interplay in serum and in adipose tissue of obese and normal-weight individuals. Diabet Metab Syndrome. 2017;9:95. DOI: 10.1186/s13098-017-0293-2.
  • Owczarz M, Budzinska M, Domaszewska-Szostek M, Borkowska J, Polosak J, Gewartowska M, Slusarczyk M, Puzianowska-Kuznicka M. miR-34a and miR-9 are overexpressed and SIRT genes are downregulated in peripheral blood mononuclear cells of aging humans. Exp Biol Med. 2017;242:1453-1461. DOI: 10.1177/1535370217720884.
  • Lapko N, Zawadka M, Polosak J, Worthen GS, Danet-Desnoyers G, Puzianowska-Kuznicka M, Laudanski K. Long-term monocyte dysfunction after sepsis in humanized mice is related to persisted activation of Macrophage-Colony Stimulation Factor (M-CSF) and demethylation of PU.1, and it can be reversed by blocking M-CSF in vitro or by transplanting naïve autologous stem cells in vivo. Front Immunol. 2017;8:401. DOI: 10.3389/fimmu.2017.00401.
  • Hirsch IB, Franek E, Mersebach H, Bardtrum L, Hermansen K. Safety and efficacy of insulin degludec/insulin aspart with bolus mealtime insulin aspart compared with standard basal-bolus treatment in people with type 1 diabetes: 1-year results from a randomized clinical trial (BOOST® T1). Diabet Med. 2017;34:167-173. DOI: 10.1111/dme.13068.
  • Budzinska M, Pawlik-Pachucka E, Owczarz M, Roszkowska-Gancarz M, Slusarczyk P, Puzianowska-Kuznicka M. miR-96 and miR-145 expression increases, and IGF-1R and FOXO1 expression decreases in peripheral blood mononuclear cells of successfully aging humans. BMC Geriatrics. 2016;16:200. DOI: 10.1186/s12877-016-0379-y.
  • Puzianowska-Kuznicka M, Owczarz M, Wieczorowska-Tobis K, Nadrowski P, Chudek J, Slusarczyk P, Skalska A, Jonas M, Franek E, Mossakowska M. Interleukin-6 and C-reactive protein, successful aging, and mortality: the PolSenior study. Immun Ageing. 2016;13:21. DOI: 10.1186/s12979-016-0076-x.
  • Kurylowicz A, Owczarz M, Polosak J, Jonas M, Lisik W, Jonas M, Chmura A, Puzianowska-Kuznicka M. SIRT1 and SIRT7 expression in adipose tissues of obese and normal-weight individuals is regulated by microRNAs but not by methylation status. Int J Obes. 2016;40:1635-1642. DOI: 10.1038/ijo.2016.131.
  • Bergenstal RM, Lunt H, Franek E, Travert F, Mou J, Qu Y, Antalis CJ, Hartman ML, Rosilio M, Jacober SJ, Bastyr EJ 3rd; IMAGINE 3 Trial Investigators. A randomized, double-blind clinical trial comparing basal insulin Peglispro and insulin Glargine, in combination with prandial insulin Lispro, in patients with type 1 diabetes: IMAGINE 3. Diabetes Obes Metab. 2016;18:1081-1088. DOI: 10.1111/dom.12698.
  • Petrie JR, Marso SP, Bain SC, Franek E, Jacob S, Masmiquel L, Leiter LA, Haluzik M, Satman I, Omar M, Shestakova M, Van Gaal L, Mann JF, Baeres FM, Zinman B, Poulter NR; LEADER investigators. LEADER-4: blood pressure control in patients with type 2 diabetes and high cardiovascular risk: baseline data from the LEADER randomized trial. J Hypertens. 2016;34:1140-1150. DOI: 10.1097/HJH.0000000000000890.
  • Hamann L, Bustami J, Iakoubov L, Szwed M, Mossakowska M, Schumann RR, Puzianowska-Kuznicka M. TLR-6 SNP P249S is associated with healthy aging in nonsmoking Eastern European Caucasians - a cohort study. Immun Ageing. 2016;13:7. DOI: 10.1186/s12979-016-0062-3.
  • Puzianowska-Kuznicka M, Walicka M, Osinska B, Rutkowski D, Gozdowski D, Czech M, Durlik M, Franek E. In-hospital mortality in a 4-year cohort study of 3,093,254 operations in seniors. World J Surg. 2016;40:1068-1074. DOI: 10.1007/s00268-015-3400-2.
  • Gajewska J, Kuryłowicz A, Ambroszkiewicz J, Mierzejewska E, Chełchowska M, Szamotulska K, Weker H, Puzianowska-Kuźnicka M. ADIPOQ -11377C>G polymorphism increases risk of adipokine abnormalities and child obesity regardless of dietary intake. J Pediatr Gastroenterol Nutr. 2016;62:122-129. DOI: 10.1097/MPG.0000000000000900.
  • Franek E, Haluzík M, Canecki Varžić S, Sargin M, Macura S, Zacho J, Christiansen JS. Twice-daily insulin degludec/insulin aspart provides superior fasting plasma glucose control and a reduced rate of hypoglycaemia compared with biphasic insulin aspart 30 in insulin-naïve adults with type 2 diabetes. Diabet Med. 2016;33:497-505. DOI: 10.1111/dme.12982.
  • Kurylowicz A, Jonas M, Lisik W, Jonas M, Wicik ZA, Wierzbicki Z, Chmura A, Puzianowska-Kuznicka M. Obesity is associated with a decrease in expression but not with the hypermethylation of thermogenesis-related genes in adipose tissues. J Transl Med. 2015;13:31. DOI: 10.1186/s12967-015-0395-2.
  • Iakoubov L, Mossakowska M, Szwed M, Puzianowska-Kuznicka M. A common copy number variation polymorphism in the CNTNAP2 gene: sexual dimorphism in association with healthy aging and disease. Gerontology. 2015;61:24-31. DOI: 10.1159/000363320.
  • Roszkowska-Gancarz M, Jonas M, Owczarz M, Kurylowicz A, Polosak J, Franek E, Slusarczyk P, Mossakowska M, Puzianowska-Kuznicka M. Age-related changes of leptin and leptin receptor variants in healthy elderly and long-lived adults. Geriatr Gerontol Int. 2015;15:365-371. DOI: 1111/ggi.12267.
  • Mossakowska M, Broczek K, Wieczorowska-Tobis K, Klich-Raczka A, Jonas M, Pawlik-Pachucka E, Safranow K, Kuznicki J, Puzianowska-Kuznicka M. Cognitive performance and functional status are the major factors predicting survival of centenarians in Poland. J Gerontol A Biol Sci Med Sci. 2014;69:1269-1275. DOI: 10.1093/gerona/glu003.
  • Iakoubov L, Mossakowska M, Szwed M, Duan Z, Sesti F, Puzianowska-Kuznicka M. A common copy number variation (CNV) polymorphism in the CNTNAP4 gene: association with aging in females. PLoS ONE. 2013;8:e79790. DOI: 10.1371/journal.pone.0079790.
  • Franek E, Osinska B, Czech M, Figat J. Mortality after surgery in Europe. Lancet. 2013;381:369-370. DOI: 10.1016/S0140-6736(13)60161-0.
  • Al-Haggar M, Madej-Pilarczyk A, Kozlowski L, Bujnicki JM, Yahia S, Abdel-Hadi D, Shams A, Ahmad N, Hamed S, Puzianowska-Kuznicka M. A novel homozygous p.Arg527Leu LMNA mutation in two unrelated Egyptian families causes overlapping mandibuloacral dysplasia and progeria syndrome. Eur J Hum Genet. 2012;20:1134-1140. DOI: 10.1038/ejhg.2012.77.
  • Puzianowska-Kuznicka M. ESR1 in myocardial infarction. Clin Chim Acta. 2012;413:81-87. DOI: 10.1016/j.cca.2011.10.028.
  • Polosak J, Kurylowicz A, Roszkowska-Gancarz M, Owczarz M, Puzianowska-Kuznicka M. Aging is accompanied by a progressive decrease of expression of the WRN gene in human blood mononuclear cells. J Gerontol A Biol Sci Med Sci. 2011;66:19-25. DOI: 1093/gerona/glq162.