Head
Katarzyna Kaczyńska, Assoc. Prof, PhD, phone: +48 22 60 86 520; This email address is being protected from spambots. You need JavaScript enabled to view it.

Research staff
Kryspin Andrzejewski, PhD- phone: +48 22 60 86 525; This email address is being protected from spambots. You need JavaScript enabled to view it.
Monika Jampolska, PhD - phone: +48 22 60 86 525; This email address is being protected from spambots. You need JavaScript enabled to view it.
Ewelina Russjan PhD - phone: +22 60 86 496; This email address is being protected from spambots. You need JavaScript enabled to view it.
Piotr Wojciechowski, PhD – phone: +48 22 60 86 464; This email address is being protected from spambots. You need JavaScript enabled to view it.
Dominika Zając, PhD - phone: +48 22 60 86 496; This email address is being protected from spambots. You need JavaScript enabled to view it.

PhD students
Ilona Mojzych, MSc (MISDoMP) - phone: +48 22 60 86 520; This email address is being protected from spambots. You need JavaScript enabled to view it. .pl
Agnieszka Wrzesień, MSc - phone: +48 22 60 86 525; This email address is being protected from spambots. You need JavaScript enabled to view it. 


Research profile
• Mechanisms of chemical and neural regulation of breathing in animal models of neurodegenerative diseases
• Role of neuropeptides and opioids in peripheral control of breathing. Cardiovascular and respiratory patterns evoked by endogenous neuropeptides and opioids. Reversal of post-opioid respiratory depression
• Investigation of anti-inflammatory and anti-constrictor potency of various chemicals in murine model of asthma

Grants

  • Effects of activation of cholinergic, noradrenergic, and serotonergic systems on respiratory impairment in Alzheimer's disease. Grant NSC 2022/45/B/NZ7/00221. Principal investigator: Katarzyna Kaczyńska (2023-2026)
  • Influence of proinflammatory cytokines IL1, IL6 and TNF-α and acute intermittent hypoxia on respiratory activity in obesity. NSC 2022/06/X/NZ5/00782. Principal investigator: Monika Jampolska (2022-2023).  
  • Exploring pro-inflammatory interleukins as major players in the link carotid body - metabolic deregulation in obesity and obstructive sleep apnea. NAWA BPN/BPT/2021/1/00063. Joint research project with Portuguese partner. Coordinator: Kryspin Andrzejewski (2022-2023)
  • Preparation of carotid body and registration of sinus nerve activity. NSC 2019/03/X/NZ4/00315. Principal investigator: Kryspin Andrzejewski (2019-2020)
  • Influence of “Wieliczka” brine inhalation on inflammatory process and bronchial reactivity in murine model of nonatopic asthma. Grant NSC 2017/01/X/NZ5/00325. Principal investigator: Dominika Zając (2017-2018)
  • Impact of intracerebroventricular and systemic injection of neuropeptide FF on breathing pattern and post-opioid depression of ventilation in anesthetized rats. NSC 2017/01/X/NZ4/00465. Principal investigator: Piotr Wojciechowski (2017-2018)
  • Impact of opioid-neurotensin chimera (PK20) on inflammation in murine model of contact sensitivity reaction and non-atopic asthma - comparison with action of neurotensin and endomorphin 2 pharmacophore. NCN 2014/13/B/NZ7/02247. Principal investigator: Katarzyna Kaczyńska (2015-2017)

Collaboration
domestic

• Department of Pharmacodynamics, Medical university of Warsaw
• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw
• Department of Chemistry, Warsaw University
• Nencki Institute of Experimental Biology PAS, Warsaw
• National Medicines Institute, Warsaw
foreign
• Nova Medical School, Lisbona, Portugalia
• Department of Pharmacy, University "G. d'Annunzio" of Chieti-Pescara, Italy

Research equipment
• pneumotachograph (Hans Rudolph inc.)
• whole body rodent plethysmograph (Buxco Electronics)
• blood pressure monitor (Columbus Instruments)
• capnograph (Engstrom eliza)
• blood gas analyzer (VetStat)
• respiratory pump (Ugo Basile)
• spectrophotometr (GBC Scientific Equipment Ltd.)
• microplate reader Epoch (BioTek Instruments)
• centrifuge cytospin 4 (Thermo Scientific)
• light microscope (Nikon)
• mikroskop świetlny (Nikon)

Research methods
• measurement of respiratory parameters in anesthetized and awake animals during hypoxia and hypercapnia
• recording of electrophysiological activity of phrenic and hypoglossal nerves (NeuroLog)
• registration of blood pressure and heart rate in anesthetized animals
• stereotacxic operations and dug injections to specific brain structures
• the experimental models used (in vivo): asthma, Parkinson’s disease, Alzheimer’s disease

Selected publications

  • Kaczyńska K, Jampolska M, Wojciechowski P, Sulejczak D, Andrzejewski K, Zając D. Potential of Lactoferrin in the treatment of lung diseases. Pharmaceuticals 2023, 16, 192
  • Andrzejewski, K.; Jampolska, M.; Mojzych, I.; Conde, S.V.; Kaczyńska, K. Hypoxic and Hypercapnic Responses in Transgenic Murine Model of Alzheimer’s Disease Overexpressing Human AβPP: The Effects of Pretreatment with Memantine and Rivastigmine. Int J Mol Sci 2022, 23, 6004
  • Kaczyńska, K.; Orłowska, M.E.; Andrzejewski, K. Respiratory Abnormalities in Parkinson’s Disease. What Do We Know from Studies in Humans and Animal Models? Int J Mol Sci 2022, 23, 3499
  • Jampolska M, Andrzejewski K, Zaremba M, Joniec-Maciejak I, Kaczyńska K. Deficiency of Biogenic Amines Modulates the Activity of Hypoglossal Nerve in the Reserpine Model of Parkinson’s Disease. Cells 2021, 10, 531
  • Russjan E, Zając D, Sulejczak D, Kleczkowska P, Kaczyńska K. Contribution of opioid and neurotensin receptors in the anti-inflammatory activity of PK20 hybrid compound in murine airways. Clin Exp Pharmacol Physiol 2021, 00: 1-9
  • Zajac D. Inhalations with thermal waters in respiratory diseases. J Ethnopharmacol 2021, 281:114505
  • Kowalczyk P, Sulejczak D, Kleczkowska P, Bukowska-Ośko I, Kucia M, Popiel M, Wietrak E, Kramkowski K, Wrzosek K, Kaczyńska K. Mitochondrial Oxidative Stress - A Causative Factor and Therapeutic Target in Many Diseases. Int J Mol Sci 2021, 22, 13384
  • Kaczyńska K, Wojciechowski P. Non-Opioid Peptides Targeting Opioid Effects. Int J Mol Sci, 2021, 22, 13619
  • Zajac D. Mineral Micronutrients in Asthma. Nutrients. 2021, 13(11):4001.
  • Kaczyńska, K.; Zając, D.; Wojciechowski, P.; Jampolska, M. Regulatory Peptides in Asthma. Int J Mol Sci 2021, 22, 13656
  • Zając D, Russjan E, Kostrzon M, Kaczyńska K. Inhalations with brine solution from the ‘Wieliczka’ Salt Mine diminish airway hyperreactivity and inflammation in a murine model of non-atopic asthma. Int J Mol Sci 2020, 21, 4798
  • Sacramento JF, Andrzejewski K, Melo BF, Ribeiro MJ, Obeso A, Conde SV. Exploring the mediators that promote carotid body dysfunction in type 2 diabetes and obesity related syndromes. Int J Mol Sci 2020, 21, 5545
  • Wojciechowski P, Kleczkowska P, Mollica A, Stefanucci A, Kaczyńska K. Vagal apnea and hypotension evoked by systemic injection of an antinociceptive analogue of endomorphin-2. Eur J Pharmacol 2020, 885, 173514
  • Krug P, Wiktorska K, Kaczyńska K, Ofiara K, Szterk A, Wysocka B, Mazur M. Sulforaphane-assisted preparation of tellurium flower-like nanoparticles. Nanotechnology 2020, 31 (5): 055603
  • Szereda-Przestaszewska M, Kaczyńska K. Serotonin and substance P: synergy or competition in the control of breathing. Auton Neurosci. 2020, 225, 102658
  • Andrzejewski K, Jampolska M, Zaremba M, Joniec-Maciejak I, Boguszewski PM, Kaczyńska K. Respiratory pattern and phrenic and hypoglossal nerve activity during normoxia and hypoxia in 6-OHDA induced bilateral model of Parkinson’s disease. J Physiol Sci 2020, 70: 16
  • Frączek K, Ferraiolo M, Hermans E, Bujalska-Zadrozny M, Kasarello K, Erdeid A, Kulik K, Kowalczyk A, Wojciechowski P, Sulejczak D, Sosnowski P, Granica S, Benyhe S, Kaczynska K, Nagraba Ł, Stolarczyk A, Cudnoch-Jedrzejewska A, Kleczkowska P. Novel opioid-neurotensin-based hybrid peptide with spinal long-lasting antinociceptive activity and a propensity to delay tolerance development. Acta Pharm Sin B, 2020, 10, 1440-1452
  • Russjan E, Kaczyńska K. Beneficial effects of neurotensin in murine model of hapten-induced asthma. Int J Mol Sci 2019, 20(20). pii: E5025
  • Russjan E, Andrzejewski K, Sulejczak D, Kleczkowska P, Kaczyńska K. Endomorphin-2 and neurotensin based chimeric peptide attenuates airway inflammation in mouse model of non-allergic asthma. Int J Mol Sci 2019, 20 (23): 5935
  • Andrzejewski K, Budzińska K, Kaczyńska K. Effect of 6-OHDA on hypercapnic ventilatory response in the rat model of Parkinson’s disease. Physiol Res 2019, 68: 285-293
  • Krug P, Mielczarek L, Wiktorska K, Kaczyńska K, Wojciechowski P, Andrzejewski K, Ofiara K, Szterk A, Mazur M. Sulforaphane-conjugated selenium nanoparticles: Towards synergistic anticancer effect. Nanotechnology 2019, 30(6):065101
  • Szereda-Przestaszewska M, Kaczyńska K. Pharmacologically evoked apnoeas. receptors and nervous pathways involved. Life Sci 2019, 217: 237-242

Patent
Pat.234708 - „Conjugates of selenium and isothiocyanate nanoparticles for use in the treatment of tumors” (2019)