Department of Neuropeptides

Head
Piotr Lipiński, PhD, MPharm, email This email address is being protected from spambots. You need JavaScript enabled to view it., phone: +48 22 60 86 480

Research staff
Prof. Aleksandra Misicka-Kęsik, DSc, PhD email: This email address is being protected from spambots. You need JavaScript enabled to view it., phone: +48 22 60 86 454
Jolanta Dyniewicz, PhD, email: This email address is being protected from spambots. You need JavaScript enabled to view it., phone: +48 22 60 86 480
Piotr Kosson, VD, PhD, email: This email address is being protected from spambots. You need JavaScript enabled to view it., phone: +48 22 60 86 454
Joanna Matalińska, PhD, email: This email address is being protected from spambots. You need JavaScript enabled to view it., phone: +48 22 60 86 455/548
Patrycja Redkiewicz, PhD, email: This email address is being protected from spambots. You need JavaScript enabled to view it., phone: +48 22 60 86 480
PhD students
Katarzyna Kosińska, MSc email: This email address is being protected from spambots. You need JavaScript enabled to view it. , phone: +48 60 86 480

Research profile
• Search for novel analgesic substances for the treatment of acute, chronic and neuropathic pain.
• Search for novel active substances with anticancer activity.

Grants
• Radiopharmaceuticals based on neurokinin-1 receptor antagonists for the diagnosis and therapy of a brain tumour - glioblastoma multiforme, 2017/25/B/NZ7/01896, led by prof. Aleksandra Misicka-Kęsik, 2018-2021.
• Development of an NK1 receptor homology model based on the data concerning interspecies differences in ligand binding, 2016/23/D/NZ7/0363, led by Piotr F.J. Lipiński, 2017-2021.

Collaboration
domestic
• Faculty of Chemistry, University of Warsaw, Warsaw
• Institute of Nuclear Chemistry and Technology, Warsaw
• National Medicines Institute, Warsaw
• Medical University of Lodz, Lodz
• Maj Institute of Pharmacology Polish Academy of Sciences, Cracow
foreign
• Biological Research Institute, Hungarian Academy of Sciences, Szeged, Hungary
• University of Arizona, Tucson, USA

Research equipment
• Preparative HPLC setup (Shimadzu)
• HPLC setup coupled to mass spectrometry (LC-MS, Shimadzu)
• 96-well harvester, Microbeta Filtermat (Perkin Elmer)
• 24-well harvester, M-24 Cell Harvester (Brandel)
• Scintillation/luminescence counter (1450 LSC & Luminescence Counter, Wallac MicroBeta TriLux, Perkin Elmer)

Research methods
• Organic synthesis (peptides and peptidomimetics)
• Receptor binding assays with radiometric methods
• In vitro assessment of cytotoxic and antiproliferative activity
• In vivo testing of antinociceptive activity in acute and chronic pain models
• Molecular modelling of ligand-receptor interactions (docking, molecular dynamics)

Selected publications

  • Halik, P.J.; Lipiński, P.F.J.; Matalińska, J.; Koźmiński, P.; Misicka, A.; Gniazdowska, E. Radiochemical Synthesis and Evaluation of Novel Radioconjugates of Neurokinin 1 Receptor Antagonist Aprepitant Dedicated for NK1R-Positive Tumors, Molecules, 2020, 25 3756. 
  • Dyniewicz, J.; Lipiński, P.F.J.; Kosson, P.; Bochyńska-Czyż, M.; Matalińska, J.; Misicka, A. Antinociceptive and cytotoxic activity of opioid peptides with hydrazone and hydrazide moieties at the C-terminus, Molecules, 2020, 25, 3429.
  • Kowalczyk, A.; Lipiński, P.F.J.; Karoń, K.; Rode, J.E.; Lyczko, K.; Dobrowolski, J.Cz.; Donten, M.; Kaczorek, D.; Poszytek, J.; Kawęcki, R.; Łapkowski, M.; Malkowska, A.; Grudziński, I.P.; Nowicka, A.M. Enantioselective sensing of (S)-Thalidomide in blood plasma with a chiral naphthalene diimide derivative, Biosens Bioelectron, 2020, 167, 112446.
  • Matalińska J.; Lipiński P.F.J.; Kotlarz A.; Misicka A. Antiproliferative effects of [D-Pro2, D-Trp7,9]-substance P and aprepitant on several cancer cell lines and their selectivity in comparison to normal cells, Folia Neuropathol, 2020, 3, in press.
  • Tymecka, D., Lipiński, P.F.J.; Kosson, P.; Misicka, A. beta2-Homo-Amino Acid Scan of Mu-Selective Opioid Tetrapeptide TAPP, Molecules, 2020, 25, 2461
  • Adamska-Bartłomiejczyk, A.; Lipiński P.F.J.; Piekielna-Ciesielska, J.; Kluczyk, A.; Janecka, A Pharmacological profile and molecular modeling of cyclic opioid analogs incorporating various phenylalanine derivatives, ChemMedChem, 2020, 15.
  • Kokocinska-Kusiak, A.; Matalińska, J.; Sacharczuk, M.; Sobczyńska, M.; Góral-Radziszewska, K.; Wileńska, B.; Misicka, A.; Jezierski, T. Can mice be trained to discriminate urine odor of conspecifics with melanoma before clinical symptoms appear? J Vet Beh 2020 doi: 10.1016/j.jveb.2020.04.004
  • Bochynska-Czyz, M.; Redkiewicz, P.; Kozlowska, H.; Matalinska, J.; Konop, M.; Kosson, P. Can keratin scaffolds be used for creating three-dimensional cell cultures? Open Medicine, 2020 15, 249 DOI: 10.1515/med-2020-0031
  • Matalińska, J.; Lipiński, P.F.J.; Kotlarz, A.; Kosson, P.; Muchowska, A.; Dyniewicz, J. Evaluation of Receptor Affinity, Analgesic Activity and Cytotoxicity of a Hybrid Peptide, AWL3020. Int J Pept Res Ther. 2020. doi:10.1007/s10989-020-10051-5
  • Fedorczyk, B.; Lipiński, P.F.J.; Puszko, A.K.; Tymecka, D.; Wilenska, B.; Dudka, W.; Perret, G.Y.; Wieczorek, R.; Misicka, A. Triazolopeptides Inhibiting the Interaction between Neuropilin-1 and Vascular Endothelial Growth Factor-165. Molecules 2019, 24, 1756.
  • Lipiński, P.; Kosson, P.; Matalińska, J.; Roszkowski, P.; Czarnocki, Z.; Jarończyk, M.; Misicka, A.; Dobrowolski, J.; Sadlej, J. Fentanyl Family at the Mu-Opioid Receptor: Uniform Assessment of Binding and Computational Analysis. Molecules 2019, 24, 740.
  • Lipiński, P.F.J.; Szűcs, E.; Jarończyk, M.; Kosson, P.; Benyhe, S.; Misicka, A.; Dobrowolski, J.C.; Sadlej, J. Affinity of fentanyl and its derivatives for the σ 1 -receptor. Medchemcomm 2019, 10, 1187.
  • Lipiński, P.F.J.; Jarończyk, M.; Dobrowolski, J.C.; Sadlej, J. Molecular dynamics of fentanyl bound to μ-opioid receptor. J. Mol. Model. 2019, 25, 144.
  • Wilenska, B.; Tymecka, D.; Włodarczyk, M.; Sobolewska-Włodarczyk, A.; Wiśniewska-Jarosińska, M.; Dyniewicz, J.; Somogyi, Á.; Fichna, J.; Misicka, A. Enkephalin degradation in serum of patients with inflammatoryboweldiseases. Pharmacol. Reports 2019, 71, 42.
  • Zatorski, H.; Salaga, M.; Zielińska, M.; Wasilewski, A.; Misicka, A.; Sacharczuk, M.; Fichna, J. High activity of endogenous opioid system protects against gastric damage development in mouse models of gastric mucosal injury. Pharmacol. Reports 2019, 71, 218.
  • Puszko, A.K.; Sosnowski, P.; Pułka-Ziach, K.; Hermine, O.; Hopfgartner, G.; Lepelletier, Y.; Misicka, A. Urea moiety as amide bond mimetic in peptide-like inhibitors of VEGF-A165/NRP-1 complex. Bioorg. Med. Chem. Lett. 2019, 29, 2493.
  • Puszko, A.K.; Sosnowski, P.; Tymecka, D.; Raynaud, F.; Hermine, O.; Lepelletier, Y.; Misicka, A. Neuropilin-1 peptide-like ligands with proline mimetics, tested using the improved chemiluminescence affinity detection method. Medchemcomm 2019, 10, 332.
  • Sowinska, M.; Morawiak, M.; Bochyńska-Czyż, M.; Lipkowski, A.; Ziemińska, E.; Zabłocka, B.; Urbanczyk-Lipkowska, Z. Molecular Antioxidant Properties and In Vitro Cell Toxicity of the p-AminobenzoicAcid (PABA) Functionalized Peptide Dendrimers. Biomolecules 2019, 9, 89.
  • Ciechanowicz, P.; Rakowska, A.; Sikora, M.; Rudnicka, L. JAK-inhibitors in dermatology: current evidence and future applications. J. Dermatolog. Treat. 2019, 30, 648.
  • Muchowska, A.; Redkiewicz, P., Różycki K., Matalińska J.; Lipiński PFJ.; Czuwara J.; Kosson P. The analgesic hybrid of dermorphin/substance P and analog of enkephalin improve wound healing in streptozotocin-induced diabetic rats. WoundRepair Regen. 2019, doi:10.1111/wrr.12775.
  • Konop, M.; Kłodzińska E.; Borowiec J.; Laskowska AK.; Czuwara J; Konieczka P.; Cieślik B.; Waraksa E.; Rudnicka L. Application of micellar electrokinetic chromatography for detection of silver nanoparticles released from wound dressing. 2019, 40, 1565.