Department of Neurotoxicology

Head
Magdalena Zielińska, PhD, Professor - phone: +48 22 60 86 470, This email address is being protected from spambots. You need JavaScript enabled to view it.
Secretariat
Dorota Rycko - phone: +48 22 60 86 417, This email address is being protected from spambots. You need JavaScript enabled to view it.

Research staff
Jan Albrecht, PhD, Professor - phone: +48 22 60 86 617; This email address is being protected from spambots. You need JavaScript enabled to view it.
Monika Szeliga, PhD - phone: +48 22 60 86 416; This email address is being protected from spambots. You need JavaScript enabled to view it.
Marta Obara-Michlewska, PhD, phone: +48 22 60 86 416; This email address is being protected from spambots. You need JavaScript enabled to view it.
Mariusz Popek, PhD - phone: +48 22 60 86 442, This email address is being protected from spambots. You need JavaScript enabled to view it.
Karolina Orzel-Gajowik , M.Sc, - phone: +48 22 60 86 442, This email address is being protected from spambots. You need JavaScript enabled to view it.
Daniel Kalinowski, PhD - phone: +48 22 60 86 416; This email address is being protected from spambots. You need JavaScript enabled to view it.
Technical staff
Małgorzata Bogacińska-Karaś, M.Sc - phone: +48 22 60 86 440/441, This email address is being protected from spambots. You need JavaScript enabled to view it.
Kamila Szumska, M.Sc - phone: +48 22 60 86 417, This email address is being protected from spambots. You need JavaScript enabled to view it.

PhD Students
Joanna Buczkowska, M.Sc. phone +48 22 60 86 442, This email address is being protected from spambots. You need JavaScript enabled to view it.
Zuzanna Sulej,M. Sc, phone +48 22 60 86 442; This email address is being protected from spambots. You need JavaScript enabled to view it.

Former Lab Members
Anna Czarnecka, PhD
Marek Pawlik, PhD
Krzysztof Milewski, PhD
Katarzyna Skowrońska, PhD
Katarzyna Dąbrowska, PhD
Ewelina Majewska, PhD
Aleksandra Nakonieczna, M.Sc UW
Katarzyna Serafin, Ing PW
Karolina Strzelczyk, Ing PW
Julia Urbanek, Ing PW
Aleksandra Mróz, Ing PW
Wiktoria Pietrowicz, student SGGW

Research profil
• Molecular and cellular mechanisms underlying hepatic encephalopathy (HE).
• Astrocytic-neuronal-cerebral capillary endothelial cell interaction in HE-induced brain edema.
• Roles of oxidative-nitrosative stress and ammonia- glutamine interplay.
• Involvement of cGMP-associated intracellular signaling.
• Glutamine and growth of malignant gliomas: role of glutaminases.

Grants
Neurotksykologia slajd ENG

  • Role of GAB glutaminase in glioblastoma. OPUS 21 grant no  2021/41/B/NZ5/01883, National Science Centre, principal investigator: PhD Monika Szeliga (2021-2025)
  • Role of SAT1 glutamine transporter in the neurotransmission balance: implications for anxiety-related disorders. OPUS 20 grant no 2020/39/B/NZ5/01962, National Science Centre, principal investigator: Prof. PhD Magdalena Zielińska (2021-2024)
  • The role of glutamine transporter sat1 (slc38a1) deficiency in excessive weight gain in mice –glutamine supplementation as therapeutic approach and implications for human protein-lipid imbalance; grant no RG 3/2020,  Foundation NUTRICIA Project principal investigator: PhD Marta Obara-Michlewska (2020-2022)
  • Role of astrocytic NMDA receptors in the regulation of glutamine synthetase activity in cultured mouse astrocytes. Attempt at defining intraastrocytic signaling pathways. PRELUDIUM 14 grant no 2017/27/N/NZ3/02819, National Science Centre, principal investigator: Msc. Ing. Katarzyna Skowrońska (2018-2022)
  • Peroxiredoxins as potential therapeutic targets in glioblastoma. OPUS 13 grant no 2017/25/B/NZ7/00388, National Science Centre, principal investigator: PhD Monika Szeliga (2018-2022)
  • Role of glutamine synthesis in the initial phase of temporal lobe epilepsy in the juvenile rat lithium-pilocarpine model: mechanism of activation of glutamine synthetase. OPUS 12 grant no 2016/23/B/NZ4/03678, National Science Centre, principal investigator: Prof. PhD Jan Albrecht (2017-2022)
  • Blood-brain barrier impairment in hepatic encephalopathy: role of micro RNA. OPUS 10 grant no 2015/19/B/NZ4/01902, National Science Centre, principal investigator: Prof. PhD Magdalena Zielińska (2016-2022)
  • The role of aquaporin 4 in cerebral edema in murine model of acute hepatic encephalopathy and regulation of aquaporin 4 expression and/or localisation by caveolin 1 and p38 MAP kinase. MINIATURA 3 grant no 2019/03/X/NZ4/00623, National Science Centre, principal investigator: PhD Marta Obara-Michlewska (2019-2020)
  • Role of Sp1 transcription factor in the regulation of astrocytic glutamine transporter SN1 expression: effect of ammonia and astrocyte-neuron interactions. PRELUDIUM 11, grant no 2016/21/N/NZ4/03759, National Science Centre principal investigator M.sc.Ing. Katarzyna Dabrowska (2017-2020)
  • Proteinkinase B signalling pathway in glioblastoma cells transfected with sequence encoding liver-type glutaminase (GAB). PRELUDIUM 12 grant no 2016/23/N/NZ5/01428, National Science Centre, principal investigator M.sc. Ing. Ewelina Majewska (2017-2019)
  • Farnesyl X receptor in the brain - a new element in the pathomechanism of acute hepatic encephalopathy. MINIATURA 1 grant no 2017/01/X/NZ4/00110, National Science Centre, principal investigator PhD Anna Czarnecka (2017-2018)
  • Changes in protein composition and electrophysiological, characteristics of glutamatergic synapse in a mouse model of acute hepatic encephalopathy: Role of the transforming growth factor β1 (TGF β1). PRELUDIUM no 2015/19/N/NZ5/02249, National Science Centre, principal investigator: M.Sc. Ing. Mariusz Popek (2016-2019)
  • Role of astrocyte glutaminase expression by inflammatory factors in hepetic encephalopathy. PRELUDIUM grant no 2014/15/N/NZ5/03634, National Science Centre , principal investigator: M.Sc. Krzysztof Milewski (2015-2017)
  • The role of assymetric dimethylarginine (ADMA) in hepathic encephalopathy: marker, mediator or modulator? OPUS grant no 2013/09/B/NZ4/00536, National Science Centre, principal investigator PhD Magdalena Zielińska (2014-2017)
  • New 1,3,4-thiazadole derivatives as inhibitors of critical steps of the glutaminę – glutamate cycle in glioblastoma cells. SONATA grant no 2013/11/D/NZ7/00925, National Science Centre, principal investigator: Phd Monika Szeliga (2014-2017)
  • Contribution of glutamine transport to brain edema and dysfunction of the glutamatergic synapse associated with hepatic encephalopathy. Grant NCBR “CORE” Pol-Nor/196190/26/2013, principal investigator: prof. Jan Albrecht (2013-2016)
  • Role of astrocytic nmda receptors in hyperammonemic brain edema accociated with hepatic encephalopathy. HARMONIA grant no 2013/08/M/NZ3/00869, National Science Centre, principal investigator: prof. Jan Albrecht (2013-2016)

Collaboration
domestic
• The Children’s Memorial Health Institute Warsaw (dr Dariusz Rokicki)
• Medical University of Lublin (prof. Waldemar A. Turski)
• Medical University of Lublin (prof. Stanisław Czuczwar)
• Institute of Pharmacology, Polish Academy of Sciences (prof. Grzegorz Hess)
• Medical University of Lublin (prof. Radosław Rola)
• University of Warmia and Mazury in Olsztyn (dr hab. Jarosław Ćwikła)
foreign
• Faculty of Life Science, University of Manchester, UK (prof. Alexei Verkhratsky)
• The Biotechnology Centre of Oslo, University of Oslo, Norway (prof. Farrukh A. Chaudhry)
• Faculty of Sciences, University of Málaga, Spain (prof. Javier Marquez)
• Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark (Prof. Helle S. Waagepetersen, Dr. Blanca I. Aldana)

Research equipment
• IDEXX Catalyst One
• HPLC: Dionex UltiMate 3000, Dionex RF2000 Fluorescent Detector, Dionex UltiMate ECD-3000RS Electrochemical Detector,
• Thermocycler Biometra
• System G-Box
• Stereotactic system Stoelting CO
• Microdialysis system, 375/D/22QM dual channel swivel, Instech Counter-Balanced Lever Ar
• In-vitro Slice Chamber System with High Resolution Temperature Controller, Campden Instruments Ltd (745 Model)
• McIlwain Tissue Chopper, Campden Instruments Ltd
• Suprafusion System SF-6, BRANDEL
• 4D-NucleofectorTM Core/X/Y Unit – Lonza
• Pinnacle 8400 Rat Tethered System with EEG/EMG/biosensor
• JuLI fluorescent microscope
• Microplate reader Bio-Rad (filters (nm): 415, 450, 480, 570, 595, 655, 750 with plate for nucleic acids quantification)

Research methods
Biochemical/ Molecular biology
• radioisotope methods: transport analysis of labeled amino acids; receptor radioligand binding
• measurement of secondary transmitters (cyclic AMP, cyclic GMP), quantification of amino acids, their derivatives and other compounds using chromatography, enzymatic activity evaluation by colorimetric and immunoenzymatic (ELISA) methods, flow cytometry
• primary cell cultures; cell co-culture systems; human cell cultures; cultures of cell lines stably expressing genes encoding selected proteins; cloning; transfection
• immunocytochemistry, immunohistochemistry for morphological visualization and qualitative analysis of selected proteins; fluorescence microscopic analysis
• animal genotyping (PCR technique); quantitative mRNA expression assays (Real Time PCR); proteins (Western Blot); fractionation of membrane and intracellular proteins
Behavioral
• tests: anxiety (plus maze), depressive behavior (FST); sensomotor (spontaneous mobility (OF, RotaRod, Griptest); Morris maze
• animal models: acute hepatic encephalopathy; bile duct ligation (BDL); simple hyperammonaemia; lithium-pilocarpine model of temporal epilepsy in rat; immobilization stress
• instrumental: stereotaxic implantation of cannula/probe into specific sites of the cerebral cortex (PFC) for compounds administration; isolation of brain structures; cerebral microperfusion/dialysis in small laboratory animals (free-running and under anesthesia)

Selected publications

  • Babiec L, Wilkaniec A, Gawinek E, Hilgier W, Adamczyk A. Inhibition of purinergic P2 receptors prevents synaptic and behavioral alterations in a rodent model of autism spectrum disorders. Elsevier. Research in Autism Spectrum Disorders 112, 2024, 102353
  • Orzeł- Gajowik K., Gajowik T., Rówienicz Ł., Zielińska M. The Ohm-azing custom-made Transendothelial Electrical Resistance measuring device (and why is it a current sensation?) Sensors and Actuators: B. Chemical 404 (2024) 135192
  • Buczkowska, J.; Szeliga, M. Two Faces of Glutaminase GLS2 in Carcinogenesis. Cancers 2023, 15, 5566. https://doi.org/10.3390/cancers15235566 
  • Orzeł-Gajowik K., Milewski K., Zielińska M. miRNA-ome plasma analysis unveils changes in blood-brain barrier integrity associated with acute liver failure in rats. Fluids Barriers CNS. 2023 Dec 8;20(1):92.  doi: 10.1186/s12987-023-00484-7
  • Szeliga M, Rola R. Conoidin A, a Covalent Inhibitor of Peroxiredoxin 2, Reduces Growth of Glioblastoma Cells by Triggering ROS Production. Cells2023, 12(15), 1934;  https://doi.org/10.3390/cells12151934 
  • Czarnecka AM, Obara-Michlewska M, Wesół-Kucharska D, Greczan M, Kaczor M, Książyk J, Rokicki D, Zielińska M. S100B Protein but Not 3-Nitrotyrosine Positively Correlates with Plasma Ammonia in Patients with Inherited Hyperammonemias: A New Promising Diagnostic Tool? J Clin Med. 2023 Mar 21;12(6):2411. doi: 10.3390/jcm12062411.
  • Pawlik M., Czarnecka A.M., Kołodziej M., Skowrońska K., Węgrzynowicz M., Podgajna M., Czuczwar S.J., Albrecht J. Attenuation of initial pilocarpine-induced electrographic seizures by methionine sulfoximine pretreatment tightly correlates with the reduction of the extracellular taurine in the hippocampus. Epilepsia, 2023, https://doi.org/10.1111/epi.17554 
  • Szeliga M, Rola R. Menadione potentiates auranofin-induced glioblastoma cell death. Int J Mol Sci. 2022 Dec 11;23(24):15712. doi: 10.3390/ijms232415712 publikacja oryginalna. Open access.
  • Obara-Michlewska M, Szeliga M. Editorial for the Neurochemistry International Special Issue "The oxidative/nitrosative stress in brain tumors".  Neurochem Int. 2022 Sep;158:105377. doi: 10.1016/j.neuint.2022.105377.
  • Sowińska M, Szeliga M, Morawiak M, Zabłocka B, Urbanczyk-Lipkowska Z Design, Synthesis and Activity of New N1-Alkyl Tryptophan Functionalized Dendrimeric Peptides against Glioblastoma. Biomolecules. 2022 Aug 13;12(8):1116. doi: 10.3390/biom12081116.
  • Milewski K., Orzeł‑Gajowik K, Zielińska  M. Mitochondrial Changes in Rat Brain Endothelial Cells Associated with Hepatic Encephalopathy: Relation to the Blood–Brain Barrier Dysfunction. Neurochemical Research Published https://doi.org/10.1007/s11064-022-03698-7

  • Zielinska M., Albrecht J., Popek M. Dysregulation of Astrocytic Glutamine Transport in Acute Hyperammonemic Brain Edema. Front Neurosci. 2022; 16: 874750. Published online 2022 Jun 6. doi: 10.3389/fnins.2022.874750.

  • Obara-Michlewska M. The tryptophan metabolism, kynurenine pathway and oxidative stress - implications for glioma pathobiology. Neurochem Int. 2022 Jun 3;158:105363. doi: 10.1016/j.neuint.2022.105363. Online ahead of print.

  • Popek M, Bobula B, Orzeł-Gajowik K, Zielińska M.   The Effect of TGF-β1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy. Int J Mol Sci. 2022 Jan 19;23(3):1081. doi: 10.3390/ijms23031081.

  • Szeliga M. Comprehensive analysis of the expression levels and prognostic values of PRDX family genes in glioma. Neurochem Int. 2021 Dec 28; 153:105256. doi: 10.1016/j.neuint.2021.105256.
  • Mielcarska MB, Skowrońska K, Wyżewski Z, Toka FN. Disrupting Neurons and Glial Cells Oneness in the Brain-The Possible Causal Role of Herpes Simplex Virus Type 1 (HSV-1) in Alzheimer's Disease. Int J Mol Sci. 2021 Dec 27;23(1):242. doi: 10.3390/ijms23010242.
  • Orzeł-Gajowik K, Milewski K, Zielińska M. Insight into microRNAs-Mediated Communication between Liver and Brain: A Possible Approach for Understanding Acute Liver Failure? Int J Mol Sci. 2021 Dec 25;23(1):224. doi: 10.3390/ijms23010224.
  • Szeliga M. Comprehensive analysis of the expression levels and prognostic values of PRDX family genes in glioma. Neurochem Int. 2021 Dec 28;153:105256. doi: 10.1016/j.neuint.2021.105256.
  • Pawlik, M.J.; Aldana, B.I.; Belfiori-Carrasco, L.F.; Obara-Michlewska, M.; Popek, M.P.; Czarnecka, A.M.; Albrecht, J. Inhibition of Glutamate Release, but Not of Glutamine Recycling to Glutamate, Is Involved in Delaying the Onset of Initial Lithium-Pilocarpine-Induced Seizures in Young Rats by a Non-Convulsive MSO Dose. Int. J. Mol. Sci. 2021, 22, 11127. https://doi.org/10.3390/ijms222011127
  • Dąbrowska K., Skowrońska K. , Popek M., Albrecht J. and Zielińska M. The Role of Nrf2 Transcription Factor and Sp1-Nrf2 Protein Complex in Glutamine Transporter SN1 Regulation in Mouse Cortical Astrocytes Exposed to Ammonia. Int. J. Mol. Sci. 2021, 22(20), 11233; https://doi.org/10.3390/ijms222011233 (registering DOI)
  • Pawlik M. J., Miziak B.,Walczak A., Konarzewska A., Chrościńska-Krawczyk M.,Albrecht J. and Czuczwar S. J. Selected Molecular Targets for Antiepileptogenesis. Int. J. Mol. Sci. 2021, 22, 9737 https://doi.org/10.3390/ijms22189737
  • Hamdani E. H., Popek M., Frontczak-Baniewicz M., Utheim T.P. , Albrecht J. Zielińska M. Chaudhry F. A. Perturbation of astroglial Slc38 glutamine transporters by NH4+ contributes to neurophysiologic manifestations in acute liver failure. The Faseb Journal 2021 doi: 10.1096/fj.202001712RR
  • Milewski K. , Czarnecka A. M. , Albrecht J. and Zielińska M. Decreased Expression and Uncoupling of Endothelial Nitric Oxide Synthase in the Cerebral Cortex of Rats with Thioacetamide-Induced Acute Liver Failure. Int. J. Mol. Sci. 2021, 22, 6662. https://doi.org/10.3390/ijms22136662
  • Sowińska M, Szeliga M, Morawiak M, Ziemińska E, Zabłocka B, Urbańczyk-Lipkowska Z. Peptide dendrimers with non-symmetric bola structure exert long term effect on glioblastoma and neuroblastoma cell lines. Biomolecules 2021, 11(3), 435; https://doi.org/10.3390/biom11030435.
  • Szeliga M, Albrecht J. Roles of nitric oxide and polyamines in brain tumor growth. Adv Med Sci. 2021 Mar 9;66(1):199-205. doi: 10.1016/j.advms.2021.02.006
  • Pawlik MJ, Obara-Michlewska M, Popek MP, Czarnecka AM, Czuczwar SJ, Łuszczki J, Kołodziej M, Acewicz A, Wierzba-Bobrowicz T, Albrecht J. Pretreatment with a glutamine synthetase inhibitor MSO delays the onset of initial seizures induced by pilocarpine in juvenile rats. Brain Res. 2021 Jan 7;147253. DOI: 10.1016/j.brainres.2020.147253 
  • Szeliga M. Peroxiredoxins in Neurodegenerative Diseases. Antioxidants (Basel). 2020 Nov 30;9(12):E1203. doi: 10.3390/antiox9121203 Praca przeglądowa. Forma dostępu: open acces.
  • Czarnecka AM ,Milewski K, Albrecht J, Zielińska M. The Status of Bile Acids and Farnesoid X Receptor in Brain and Liver of Rats with Thioacetamide-Induced Acute Liver Failure. Int. J. Mol. Sci. 2020, 21(20), 7750; https://doi.org/10.3390/ijms21207750
  • Szeliga M, Karpińska M, Rola R, Niewiadomy A. Design, synthesis and biological evaluation of novel 1,3,4-thiadiazole derivatives as anti-glioblastoma agents targeting the AKT pathway. Bioorg Chem. 2020 Oct 9;105:104362. doi: 10.1016/j.bioorg.2020.104362
  • Skowrońska K, Kozłowska H, Albrecht J. Neuron-derived factors negatively modulate ryanodine receptor-mediated calcium release in cultured mouse astrocytes. Cell Calcium. 2020. 92: 102304. doi: 10.1016/j.ceca.2020.102304
  • Szeliga M. Thiadiazole derivatives as anticancer agents. Pharmacol Rep. Sep 3. doi: 10.1007/s43440-020-00154-7 Online ahead of print.
  • Popek M, Bobula B, Sowa J, Hess G, Frontczak-Baniewicz M, Albrecht J, Zielińska M. Physiology and morphological correlates of excitatory transmission are preserved in glutamine transporter SN1- depleted mouse frontal cortex. Neuroscience 2020 August 26 https://doi.org/10.1016/j.neuroscience.2020.08.019
  • Czarnecka AM, Hilgier W, Zielińska M. S-Adenosylmethionine Deficiency and Brain Accumulation of S-Adenosylhomocysteine in Thioacetamide-Induced Acute Liver Failure. Nutrients 2020, 12(7), 2135; https://doi.org/10.3390/nu12072135
  • Czarnecka A, Zielińska M. Po co nam kwasy żółciowe w mózgu? KOSMOS Vol. 69, 1, 37–44, 2020 https://kosmos.ptpk.org/index.php/Kosmos/issue/view/150
  • Obara-Michlewska M, Szeliga M. Targeting Glutamine Addiction in Gliomas. Cancers (Basel). 2020 Jan 29;12(2). pii: E310. doi: 10.3390/cancers12020310.
  • Milewski K, Bogacińska-Karaś M, Hilgier W, Albrecht J, Zielińska M. TNFα increases STAT3-mediated expression of glutaminase isoform KGA in cultured rat astrocytes. Cytokine. 2019 Jul 22;123:154774. doi: 10.1016/j.cyto.2019.154774. [Epub ahead of print]
  • Albrecht J, Zielińska M. Exchange-mode glutamine transport across CNS cell membranes. Neuropharmacology. 2019 Mar 8. pii: S0028-3908(19)30081-4. doi: 10.1016/j.neuropharm.2019.03.003. [Epub ahead of print]
  • Majewska E, Márquez J, Albrecht J, Szeliga M. Transfection with GLS2 Glutaminase (GAB) Sensitizes Human Glioblastoma Cell Lines to Oxidative Stress by a Common Mechanism Involving Suppression of the PI3K/AKT Pathway. Cancers (Basel) 2019 Jan 19;11(1). pii: E115. doi: 10.3390/cancers11010115.
  • Skowrońska K, Obara-Michlewska M, Zielińska M, Albrecht J. NMDA Receptors in Astrocytes: In Search for Roles in Neurotransmission and Astrocytic Homeostasis. Int J Mol Sci. 2019 Jan 14;20(2). pii: E309. doi: 10.3390/ijms20020309.
  • Dąbrowska K, Zielińska M Silencing of Transcription Factor Sp1 Promotes SN1 Transporter Regulation by Ammonia in Mouse Cortical Astrocytes. Int J Mol Sci. 2019 Jan 9;20(2). pii: E234. doi: 10.3390/ijms20020234.
  • Skowrońska K, Obara-Michlewska M, Czarnecka A, Dąbrowska K, Zielińska M, Albrecht J. Persistent Overexposure to N-Methyl-D-Aspartate (NMDA) Calcium-Dependently Downregulates Glutamine Synthetase, Aquaporin 4, and Kir4.1 Channel in Mouse Cortical Astrocytes. Neurotox Res. 2019 Jan;35(1):271-280. doi: 10.1007/s12640-018-9958-3. Epub 2018 Sep 15.
  • Czarnecka A, Aleksandrowicz M, Jasiński K, Jaźwiec R, Kalita K, Hilgier W, Zielińska M. Cerebrovascular reactivity and cerebral perfusion of rats with acute liver failure: role of L-glutamine and asymmetric dimethylarginine in L-arginine-induced response. J Neurochem. 2018 Dec;147(5):692-704. doi: 10.1111/jnc.14578. Epub 2018 Nov 6.
  • Dąbrowska K, Albrecht J, Zielińska M. Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes. Neurochem Int. 2018 Sep;118:225-232. doi: 10.1016/j.neuint.2018.07.001. Epub 2018 Jul 3.
  • Dabrowska K, Skowronska K, Popek M, Obara-Michlewska M, Albrecht J, Zielinska M. Roles of Glutamate and Glutamine Transport in Ammonia Neurotoxicity: State of the Art and Question Marks. Endocr Metab Immune Disord Drug Targets. 2018;18(4):306-315. doi: 10.2174/1871520618666171219124427.
  • Obara-Michlewska M, Ding F, Popek M, Verkhratsky A, Nedergaard M, Zielinska M, Albrecht J. Interstitial ion homeostasis and acid-base balance are maintained in oedematous brain of mice with acute toxic liver failure. Neurochem Int. 2018 Sep;118:286-291. doi: 10.1016/j.neuint.2018.05.007. Epub 2018 May 14.
  • Popek M, Bobula B, Sowa J, Hess G, Polowy R, Filipkowski RK, Frontczak-Baniewicz M, Zabłocka B, Albrecht J, Zielińska M. Cortical Synaptic Transmission and Plasticity in Acute Liver Failure Are Decreased by Presynaptic Events. Mol Neurobiol. 2018 Feb;55(2):1244-1258. doi: 10.1007/s12035-016-0367-4. Epub 2017 Jan 23.
  • Milewski K, Bogacińska-Karaś M, Fręśko I, Hilgier W, Jaźwiec R, Albrecht J, Zielińska M. Ammonia Reduces Intracellular Asymmetric Dimethylarginine in Cultured Astrocytes Stimulating Its y⁺LAT2 Carrier-Mediated Loss. Int J Mol Sci. 2017 Nov 2;18(11). pii: E2308. doi: 10.3390/ijms18112308.
  • Majewska E, Rola R, Barczewska M, Marquez J, Albrecht J, Szeliga M. Transcription factor GATA3 expression is induced by GLS2 overexpression in a glioblastoma cell line but is GLS2-independent in patient-derived glioblastoma. J Physiol Pharmacol. 2017 Apr;68(2):209-214.
  • Zielińska M, Aschner M. Enthusiasm Scientifically Oriented: The Preface for the Special Issue Dedicated to Jan Albrecht. Neurochem Res. 2017 Mar;42(3):711-712. doi: 10.1007/s11064-016-2164-x.
  • Czarnecka A, Milewski K, Zielińska M. Asymmetric Dimethylarginine and Hepatic Encephalopathy: Cause, Effect or Association? Neurochem Res. 2017 Mar;42(3):750-761. doi: 10.1007/s11064-016-2111-x. Epub 2016 Nov 25.
  • Majewska E, Szeliga M. AKT/GSK3β Signaling in Glioblastoma. Neurochem Res. 2017 Mar;42(3):918-924. doi: 10.1007/s11064-016-2044-4. Epub 2016 Aug 27.
  • Czarnecka A, Milewski K, Jaźwiec R, Zielińska M. Intracerebral Administration of S-Adenosylhomocysteine or S-Adenosylmethionine Attenuates the Increases in the Cortical Extracellular Levels of Dimethylarginines Without Affecting cGMP Level in Rats with Acute Liver Failure. Neurotox Res. 2017 Jan;31(1):99-108. doi: 10.1007/s12640-016-9668-7. Epub 2016 Sep 8.
  • Zielińska M, Dąbrowska K, Hadera MG, Sonnewald U, Albrecht J. System N transporters are critical for glutamine release and modulate metabolic fluxes of glucose and acetate in cultured cortical astrocytes: changes induced by ammonia. J Neurochem. 2016 Jan;136(2):329-38. doi: 10.1111/jnc.13376. Epub 2015 Nov 10.
  • Milewski K, Hilgier W, Fręśko I, Polowy R, Podsiadłowska A, Zołocińska E, Grymanowska AW, Filipkowski RK, Albrecht J, Zielińska M. Carnosine Reduces Oxidative Stress and Reverses Attenuation of Righting and Postural Reflexes in Rats with Thioacetamide-Induced Liver Failure. Neurochem Res. 2016 Feb;41(1-2):376-84. doi: 10.1007/s11064-015-1821-9. Epub 2016 Jan 22. 
  • Szeliga M, Ćwikła J, Obara-Michlewska M, Cichocki A, Albrecht J. Glutaminases in slowly proliferating gastroenteropancreatic neuroendocrine neoplasms/tumors (GEP-NETs): Selective overexpression of mRNA coding for the KGA isoform. Exp Mol Pathol. 2016 Feb;100(1):74-8. doi: 10.1016/j.yexmp.2015.11.017. Epub 2015 Nov 12.
  • Szeliga M, Różycka A, Jędrak P, Barańska S, Janik P, Jamrozik Z, Albrecht J. Expression of RNAs Coding for Metal Transporters in Blood of Patients with Huntington's Disease. Neurochem Res. 2016 Feb;41(1-2):101-6. doi: 10.1007/s11064-015-1737-4. Epub 2015 Oct 15.
  • Szeliga M, Bogacińska-Karaś M, Kuźmicz K, Rola R, Albrecht J. Downregulation of GLS2 in glioblastoma cells is related to DNA hypermethylation but not to the p53 status. Mol Carcinog. 2016 Sep;55(9):1309-16. doi: 10.1002/mc.22372. Epub 2015 Aug 10.
  • Albrecht J, Zielińska M. Mechanisms of Excessive Extracellular Glutamate Accumulation in Temporal Lobe Epilepsy. Neurochem Res. 2017 Jun;42(6):1724-1734. doi: 10.1007/s11064-016-2105-8. Epub 2016 Nov 21.
  • Milewski K, Oria M. What we know: the inflammatory basis of hepatic encephalopathy. Metab Brain Dis. 2016 Dec;31(6):1239-1247. Epub 2015 Oct 26.
  • Jęśko H, Wilkaniec A, Cieślik M, Hilgier W, Gąssowska M, Lukiw WJ, Adamczyk A1.Altered Arginine Metabolism in Cells Transfected with Human Wild-Type Beta Amyloid Precursor Protein (βAPP). Curr Alzheimer Res. (2016) 13(9):1030-9.
  • Zielińska M, Milewski K, Skowrońska M, Gajos A, Ziemińska E, Beręsewicz A, Albrecht J. Induction of inducible nitric oxide synthase expression in ammonia-exposed cultured astrocytes is coupled to increased arginine transport by upregulated y(+)LAT2 transporter. J Neurochem. 2015 Dec;135(6):1272-81. doi: 10.1111/jnc.13387. Epub 2015 Nov 6.
  • E. Zieminska, A. Stafiej, B. Toczylowska B, J. Albrecht, JW. Łazarewicz. Role of Ryanodine and NMDA Receptors in Tetrabromobisphenol A-Induced Calcium Imbalance and Cytotoxicity in Primary Cultures of Rat Cerebellar Granule Cells. Neurotox Res. 2015 Oct;28(3):195-208. doi: 10.1007/s12640-015-9546-8.
  • Milewski K, Hilgier W, Albrecht J, Zielińska M.The dimethylarginine (ADMA)/nitric oxide pathway in the brain and periphery of rats with thioacetamide-induced acute liver failure: Modulation by histidine. Neurochem Int. 2015 Sep;88:26-31. doi: 10.1016/j.neuint.2014.12.004. Epub 2014 Dec 15.
  • Ruszkiewicz J, Albrecht J.Changes of the thioredoxin system, glutathione peroxidase activity and total antioxidant capacity in rat brain cortex during acute liver failure: modulation by L-histidine. Neurochem Res. 2015 Feb;40(2):293-300. doi: 10.1007/s11064-014-1417-9. Epub 2014 Aug 27.
  • Szeliga M, Albrecht J Opposing roles of glutaminase isoforms in determining glioblastoma cell phenotype. Neurochem Int. 2015 Sep;88:6-9. doi: 10.1016/j.neuint.2014.11.004. Epub 2014 Dec 18.
  • Ruszkiewicz J, Albrecht J Changes in the mitochondrial antioxidant systems in neurodegenerative diseases and acute brain disorders. Neurochem Int. 2015 Sep;88:66-72. doi: 10.1016/j.neuint.2014.12.012. Epub 2015 Jan 6.
  • Obara-Michlewska M, Ruszkiewicz J, Zielińska M, Verkhratsky A, Albrecht J Astroglial NMDA receptors inhibit expression of Kir4.1 channels in glutamate-overexposed astrocytes in vitro and in the brain of rats with acute liver failure. Neurochem Int. 2015 Sep;88:20-5. doi: 10.1016/j.neuint.2014.10.006. Epub 2014 Oct 23.
  • Albrecht J, Zielińska M. Deficit of astroglia-derived thrombospondin-1 and loss of synaptic proteins in hepatic encephalopathy: do ammonia-overexposed astrocytes derange the synaptic hardware? J Neurochem. 2014 Nov;131(3):265-7. doi: 10.1111/jnc.12823. Epub 2014 Aug 8.
  • Zielińska M, Obara-Michlewska M, Hilgier W, Albrecht J.Citrulline uptake in rat cerebral cortex slices: modulation by Thioacetamide -Induced hepatic failure. Metab Brain Dis. 2014 Dec;29(4):1053-60. doi: 10.1007/s11011-013-9472-5. Epub 2014 Jan 3.
  • J. Albrecht (2014) Ammonia. In: Aminoff M.J. and Daroff R.B. (eds.) Encyclopedia of the Neurological Sciences, 2nd edition, vol. 1, pp. 148-151. Oxford: Academic Press.
  • Hilgier W, Kocki T, Obara-Michlewska M, Turski WA, Oja SS, Saransaari P, Albrecht J. Modulation by kynurenine of extracellular kynurenate and glutamate in cerebral cortex of rats with acute liver failure. Pharmacol Rep.2014 Jun;66(3):466-70. doi: 10.1016/j.pharep.2013.11.007. Epub 2014 Apr 13.
  • Zielińska M, Popek M, Albrecht J. Roles of changes in active glutamine transport in brain edema development during hepatic encephalopathy: an emerging concept. Neurochem Res. 2014;39(3):599-604. doi: 10.1007/s11064-013-1141-x. Epub 2013 Sep 26.
  • Martín-Rufián M, Nascimento-Gomes R, Higuero A, Crisma AR, Campos-Sandoval JA, Gómez-García MC, Cardona C, Cheng T, Lobo C, Segura JA, Alonso FJ, Szeliga M, Albrecht J, Curi R, Márquez J, Colquhoun A, Deberardinis RJ, Matés JM. Both GLS silencing and GLS2 overexpression synergize with oxidative stress against proliferation of glioma cells. J Mol Med (Berl) 2014 Mar;92(3):277-90. doi: 10.1007/s00109-013-1105-2. Epub 2013 Nov 26.
  • Szeliga M, Bogacińska-Karaś M, Różycka A, Hilgier W, Marquez J, Albrecht J. Silencing of GLS and overexpression of GLS2 genes cooperate in decreasing the proliferation and viability of glioblastoma cells. Tumour Biol. 2014 Mar;35(3):1855-62. doi: 10.1007/s13277-013-1247-4. Epub 2013 Oct 6.
  • Obara-Michlewska M, Tuszyńska P, Albrecht J. Ammonia upregulates kynurenine aminotransferase II mRNA expression in rat brain: a role for astrocytic NMDA receptors? Metab Brain Dis. 2013 Jun;28(2):161-5. doi: 10.1007/s11011-012-9353-3. Epub 2012 Nov 8.
  • Ruszkiewicz J, Fręśko I, Hilgier W, Albrecht J. Decrease of glutathione content in the prefrontal cortical mitochondria of rats with acute hepatic encephalopathy: prevention by histidine. Metab Brain Dis. 2013 Mar;28(1):11-4. doi: 10.1007/s11011-012-9342-6. Epub 2012 Oct 20.
  • Skowrońska M, Albrecht J. Oxidative and nitrosative stress in ammonia neurotoxicity. Neurochem Int. 2013 Apr;62(5):731-7. doi: 10.1016/j.neuint.2012.10.013. Epub 2012 Nov 7.
  • Szeliga M, Zgrzywa A, Obara-Michlewska M, Albrecht J.Transfection of a human glioblastoma cell line with liver-type glutaminase (LGA) down-regulates the expression of DNA-repair gene MGMT and sensitizes the cells to alkylating agents. J Neurochem. 2012 Nov;123(3):428-36. doi: 10.1111/j.1471-4159.2012.07917.x. Epub 2012 Sep 21.
  • Juszczak M, Matysiak J, Szeliga M,Pożarowski P, Niewiadomy A, Albrecht J, Rzeski W. 2-Amino-1,3,4-thiadiazole derivative (FABT) inhibits the extracellular signal-regulated kinase pathway and induces cell cycle arrest in human non-small lung carcinoma cells. Bioorg Med Chem Lett. 2012 Sep 1;22(17):5466-9. doi: 10.1016/j.bmcl.2012.07.036. Epub 2012 Jul 20.
  • Skowrońska M, Zielińska M,Wójcik-Stanaszek L, Ruszkiewicz J, Milatovic D, Aschner M, Albrecht J. Ammonia increases paracellular permeability of rat brain endothelial cells by a mechanism encompassing oxidative/nitrosative stress and activation of matrix metalloproteinases. J Neurochem. 2012 Apr;121(1):125-34. doi: 10.1111/j.1471-4159.2012.07669.x. Epub 2012 Feb 15.
  • Zielińska M, Skowrońska M, Fręśko I, Albrecht J. Upregulation of the heteromeric y(+)LAT2 transporter contributes to ammonia-induced increase of arginine uptake in rat cerebral cortical astrocytes. Neurochem Int. 2012 Sep;61(4):531-5. doi: 10.1016/j.neuint.2012.02.021. Epub 2012 Feb 27.
  • Skowrońska M, Albrecht J. Alterations of blood brain barrier function in hyperammonemia: an overview. Neurotox Res. 2012 Feb;21(2):236-44. doi: 10.1007/s12640-011-9269-4. Epub 2011 Aug 27.
  • Hilgier W, Łazarewicz JW, Strużynska L, Frontczak-Baniewicz M, Albrecht J. Repeated exposure of adult rats to Aroclor 1254 induces neuronal injury and impairs the neurochemical manifestations of the NMDA receptor-mediated intracellular signaling in the hippocampus. Neurotoxicology. 2012 Jan;33(1):16-22. doi: 10.1016/j.neuro.2011.10.005. Epub 2011 Oct 21.