Head
prof. Ewa Koźniewska-Kołodziejska, PhD, MSc; phone: + 48 22 60 86 489, This email address is being protected from spambots. You need JavaScript enabled to view it.

Research staff
Marta Aleksandrowicz, PhD, MSc; phone: +48 22 60 86 566, +48 22 60 86 424, This email address is being protected from spambots. You need JavaScript enabled to view it.
Żanna Pastuszak-Stępień, MD, PhD; phone: +48 22 60 86 566, This email address is being protected from spambots. You need JavaScript enabled to view it.

Technical staff
Łukasz Przykaza, MSc; phone: +48 22 60 86 423, +48 22 60 86 596, This email address is being protected from spambots. You need JavaScript enabled to view it.

Research profile
• Disturbances of cerebral circulation following experimental ischemia and reperfusion.
• Pharmacological protection of the brain and cerebral blood vessels against transient ischemia/reperfusion damage
• Effect of high sodium diet and sodium-dependent hypertension on the regulation of the peripheral and cerebral resistance vessels
• Impairment of the regulation of cerebral vessels and brain function in experimental hyponatremia
• Analysis of the disturbances of intracranial pressure-volume relation in patients
• Differential diagnostics of normal pressure hydrocephalus and brain atrophy
• Effect of transcranial magnetic stimulation on blood supply and activity of the cerebral cortex in patients in acute stage of ischemic stroke

Grants

• "Searching for the background underlying the neuroprotective effects of the specific agonist of Y2 receptors - NPY(13-36) in the model of transient, focal cerebral ischemia in rats".”, nr 2019/33/N/NZ47/02894, Łukasz Przykaza, 2020 -2022.
• “Impact of vascular component and component related to cerebrospinal fluid circulation on the brain compliance”, 2016/23/N/ST7/01364, Katarzyna Kaczmarska, 2017-2019.
• „Effect of acute hyponatremia associated with vasopressin on the tone of the penetrating arterioles and the middle cerebral artery of the rat”, 2017/01/X/N24/016022017 , Marta Aleksandrowicz, 2017-2018

Collaboration
domestic

• Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw
• Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw
• Department of Neurology, Military Medical Institute, Warsaw
• Department of Neurobiology, Institute of Pharmacology PAS, Krakow
• Laboratory of Animal Models, Nencki Institute of Experimental Biology PAS, Warsaw
• Laboratory of Biomedical Optics, Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw
• Department of Neurosurgery, Medical Faculty, Medical University of Warsaw, Warsaw
• Department of Physics of Nanostructures and Nanotechnology, institute of Physics, Jagiellonian University, Krakow
foreign
• Department of Clinical Neurosciences University of Cambridge, Cambridge, UK.

Research equipment
• Two-channels laser-Doppler flowmeter (MOOR Instruments DRT4) with data acquisition station
• Setup for isolated blood vessels studies (Olympus inverted microscope, Masterflex, peristaltic pump, organ chamber)
• Gas anaesthesia system for small animals (Stoelting)
• Small animals respirator (Harvard Apparatus)
• Programmable syringe infusion pump (Harvard Apparatus)
• Blood pressure gauge (Statham-Gould)
• Homeothermic blanket system (Harvard Apparatus)
• Transcranial Doppler (Sonomed)
• Intraoperative ultrasonography with microprobe (Sonomed)
• Posturography platform

Research methods
• Measurement of microflow in cerebral cortex
• Assessment of the reactivity of isolated blood vessels
• Assessment of the neurovascular coupling
• Behavioural tests
• Lumbar infusion test
• Instrumental assessment of gait, posturography
• Noninvasive estimation of cerebral perfusion and metabolism in humans

Selected publications

  • Aleksandrowicz M, Kozniewska E. Compromised regulation of the rat brain parenchymal arterioles in vasopressin-associated acute hyponatremia. Microcirculation. 2020 Jun 30. DOI: 10.1111/micc.12644.
  • Uryga A, Kaczmarska K, Burzyńska M, Czosnyka M, Kasprowicz M. A comparison of the time constant of the cerebral arterial bed using invasive and non-invasive arterial blood pressure measurements. Physiol Meas. 2020 Jun 11. DOI: 10.1088/1361-6579/ab9bb6.
  • Kaczmarska K, Uryga A, Placek MM, Calviello L, Kasprowicz M, Varsos GV, Czosnyka Z, Koźniewska E, Sierzputowski T, Koszewski W, Czosnyka M. Critical closing pressure during experimental intracranial hypertension: comparison of three calculation methods. Neurol Res. 2020;42(5):387-397. DOI: 10.1080/01616412.2020.1733323.
  • Onyszkiewicz M, Gawrys-Kopczynska M, Sałagaj M, Aleksandrowicz M, Sawicka A, Koźniewska E, Samborowska E, Ufnal M. Valeric acid lowers arterial blood pressure in rats. Eur J Pharmacol. 2020; 877:173086. DOI: 10.1016/j.ejphar.2020.173086.
  • Aleksandrowicz M, Klapczynska K, Kozniewska E. Dysfunction of the endothelium and constriction of the isolated rat's middle cerebral artery in low sodium environment in the presence of vasopressin. Clin Exp Pharmacol Physiol. 2020;47(5):759-764. DOI: 10.1111/1440-1681.13242.
  • Onyszkiewicz M, Gawrys-Kopczynska M, Konopelski P, Aleksandrowicz M, Sawicka A, Koźniewska E, Samborowska E, Ufnal M. Butyric acid, a gut bacteria metabolite, lowers arterial blood pressure via colon- vagus nerve signaling and GPR41/43 receptors. Pflugers Arch. 2019; 471(11-12):1441-1453. DOI: 10.1007/s00424-019-02322-y
  • Bejm K, Wojtkiewicz S, Sawosz P, Perdziak M, Pastuszak Ż, Sudakou A, Guchek P, Liebert A. Influence of contrast-reversing frequency on the amplitude and spatial distribution of visual cortex hemodynamic responses. Biomed Optic Express. 2019; 12:6296-6312. DOI: 10.1364/BOE.10.006296.
  • Uryga A, Kasprowicz M, Calviello L, Diehl RR, Kaczmarska K, Czosnyka M. Assessment of cerebral hemodynamic parameters using pulsatile versus non-pulsatile cerebral blood outflow models. J Clin Monit Comput. 2019, 33(1):85-94. DOI: 10.1007/s10877-018-0136-1.
  • Uryga A, Kasprowicz M, Burzynska M, Calviello L, Kaczmarska K, Czosnyka M. Cerebral arterial time constant calculated from the middle and posterior cerebral arteries in healthy subjects. J Clin Monit Comput. 2019, 33 (4): 605-613. DOI: 10.1007/s10877-018-0207-3.
  • Czarnecka A, Aleksandrowicz M, Jasiński K, Jaźwiec R, Kalita K, Hilgier W, Zielińska M. Cerebrovascular reactivity and cerebral perfusion of rats with acute liver failure: role of L-glutamine and asymmetric dimethylarginine in L-arginine-induced response. J Neurochem. 2018; 147(5):692-704. DOI: 10.1111/jnc.14578.
  • Motyl J, Przykaza L, Boguszewski PM, Kosson P, Strosznajder JB. Pramipexole and Fingolimod exert neuroprotection in a mouse model of Parkinson's disease by activation of sphingosine kinase 1 and Akt kinase. Neuropharmacology. 2018, 135:139-150. DOI: 10.1016/j.neuropharm.2018.02.023.
  • Domin H, Przykaza L, Kozniewska E, Boguszewski PM, Śmialowska M. Neuroprotective effect of the group III mGlu receptor agonist ACPT-I after ischemic stroke in rats with essential hypertension. Prog Neuropsychopharmacol Biol Psychiatry. 2018; 84 (Pt A): 93-101. DOI: 10.1016/j.pnpbp.2018.02.006.
  • Aleksandrowicz M, Kozniewska E. Effect of vasopressin-induced chronic hyponatremia on the regulation of the middle cerebral artery of the rat. Pflugers Arch. 2018; 470 (7): 1047-1054. DOI: 10.1007/s00424-018-2141-0.
  • Pastuszak Z, Stępień A, Kamiński G, Deptuła-Krawczyk E, Trawińska L, Kryszak A. Analysis of growth hormone levels in the blood of patients with drug resistant depression. Pol Arch Intern Med. 2018 ;128(4):263-265. DOI: 10.20452/pamw.4256.
  • Pastuszak Ż, Koźniewska E, Stępień A, Piusińska-Macoch A, Czernicki Z, Koszewski W. Importance rating of risk factors of ischemic stroke in patients over 85 years old in the polish population. Neurol Neurochir Pol. 2018; 52(1):88-93 . DOI: 10.1016/j.pjnns.2017.11.007.
  • Kaczmarska K, Kasprowicz M, Uryga A, Calviello L, Varsos G, Czosnyka Z, Czosnyka M. Critical closing pressure during controlled increase in intracranial pressure – Comparison of three methods. IEEE Trans Biomed Eng. 2018, 65(3):619-624. DOI: 10.1109/TBME.2017.2707547.
  • Aleksandrowicz M, Dworakowska B, Dolowy K, Kozniewska E. Restoration of the response of the middle cerebral artery of the rat to acidosis in hyposmotic hyponatremia by the opener of large-conductance calcium sensitive potassium channels (BKCa). J Cereb Blood Flow Metab. 2017; 37 (9): 3219-3230. DOI: 10.1177/0271678X16685575.
  • Domin H, Przykaza Ł, Jantas D, Kozniewska E, Boguszewski PM, Śmiałowska M. Neuropeptide Y Y2 and Y5 receptors as promising targets for neuroprotection in primary neurons exposed to oxygen-glucose deprivation and in transient focal cerebral ischemia in rats. Neuroscience. 2017, 344:305-325. DOI: 10.1016/j.neuroscience.2016.12.040.
  • Domin H, Przykaza Ł, Jantas D, Kozniewska E, Boguszewski PM, Śmiałowska M. Neuroprotective potential of the group III mGlu receptor agonist ACPT-I in animal models of ischemic stroke: In vitro and in vivo studies. Neuropharmacology. 2016, 102:276-294. DOI: 10.1016/j.neuropharm.2015.11.025.