Head
• Jakub Godlewski PhD; phone 22 60 86 567; This email address is being protected from spambots. You need JavaScript enabled to view it.
Research staff
• Sylwia K Król PhD, phone 22 60 86 468; This email address is being protected from spambots. You need JavaScript enabled to view it.
• Joanna Reszczyńska PhD, phone 22 60 86 468, This email address is being protected from spambots. You need JavaScript enabled to view it.
• Barbara Olejniczak MSc, phone 22 60 86 468, This email address is being protected from spambots. You need JavaScript enabled to view it.
PhD students
• Adrian Szczepaniak MSc, phone 22 60 86 468; This email address is being protected from spambots. You need JavaScript enabled to view it.
Research profile
• The role of non-coding RNAs (ncRNAs): microRNA, lncRNA and circular RNA in the pathogenesis of human gliomas.
• Potential applications of ncRNAs in glioma therapy.
• Glioblastoma stem-like cells as a potential therapeutic target.
• Use of extracellular vesicles as carriers of therapeutic molecules.
Grants
• “Extracellular vesicles carrying microRNA in experimental immunotherapy for glioblastoma” - NCN OPUS 21, No. 2021/41/B/NZ6/0243, Principal Investigator: Jakub Godlewski PhD, 2022-2025
• “The role of circular RNA – MALAT1 in breast-to-brain cancer metastasis” - NCN PRELUDIUM 20, No: 2021/41/N/NZ3/02209, Principal Investigator – Adrian Szczepaniak MSc, 2022-2024
• “Control of microRNAome maturation and its application in targeted therapy of cancer stem cells” - NCN OPUS 20, No: 2020/39/B/NZ5/02893, Principal Investigator: Jakub Godlewski PhD, 2021-2024
• “MicroRNA-carrying extracellular vesicles for the therapy of glioblastoma” - Internal Research Fund IMDiK PAN 2021, Principal Investigator: Jakub Godlewski PhD, Funding: M. Mossakowski Institute of Experimental and Clinical Medicine of the Polish Academy of Sciences, 2021-2022
• “Exosome-mediated enhancement of immunotherapy against glioblastoma – the role of non-coding RNA” - National Agency for Academic Exchange, Polish Returns Programme 2019, No: PPN/PPO/2019/1/00001, Principal Investigator: Jakub Godlewski PhD, 2020-2024
Collaboration
domestic
• Elżbieta Salińska, Mossakowski Medical Research Institute PAS, Warsaw, Poland
foreign
• E. Antonio Chiocca, Brigham and Women’s Hospital, Boston, MA, USA
• Ralph Weissleder, Massachusetts General Hospital, Boston, MA, USA
• Hiroshi Nakashima, Harvard Medical School, Boston, MA, USA
• Franz Ricklefs, Medical Center Hamburg-Eppendorf, Hamburg, Germany
• Al Charest, Beth Israel Hospital, Harvard Medical School, Boston, MA, USA
• Mariano Viapiano, SUNY Upstate Medical University,Syracuse, NY, USA
• Benjamin Purow, University of Virginia, Charlottesville, VA, USA
Research methods
• Proteomics (immunodetection by Western Blotting, immunoprecipitation immunofluorescence, immunohistochemistry, mass spectroscopy).
• Genomics (quantitative RT-qPCR, microarrays, next generation sequencing NGS).
• Human cancer cell models (3D cultures of glioma stem cells)
• Animal models (intracranial tumors).
• Genetic engineering (transient or stable silencing/amplification of gene expression using siRNA, shRNA, CRISPR/Cas9 genome editing, lentiviral vectors).
• Microscopy (light, fluorescence).
• Therapeutic vehicles (nanoparticles, oncolytic viruses, extracellular vesicles).
Selected publications
2022
• Szczepaniak A., Machelak W., Fichna J., Zielińska M*. „The role of kappa opioid receptors in immune system – an overview”, European Journal of Pharmacology, 2022, 933, 175214,
• Jurkiewicz M., Steć G., Szczepaniak A., Fichna J., Zielińska M*. „GPR18 receptor – the structure and the role in the physiology and pathophysiology”, Postępy Biochemii, 2022, 68(2), 161-168,
• Fornalski, K. W.; Adamowski, Ł.; Bugała, E.; Jarmakiewicz, R.; Kirejczyk, M.; Kopyciński, J.; Krasowska, J.; Kukulski, P.; Piotrowski, Ł.; Ponikowska, J.; Reszczyńska, J.; Słonecka, I.; Wysocki, P.; Dobrzyński, L. Biophysical Modeling of the Ionizing Radiation Influence on Cells Using the Stochastic (Monte Carlo) and Deterministic (Analytical) Approaches. Dose-Response. 2022,20(4), doi:10.1177/15593258221138506.
2021
• Sławińska-Brych, A.; Mizerska-Kowalska, M.; Król, S.K.; Stepulak, A.; Zdzisińska, B. Xanthohumol Impairs the PMA-Driven Invasive Behaviour of Lung Cancer Cell Line A549 and Exerts Anti-EMT Action. Cells 2021, 10, 1484, doi:10.3390/cells10061484.
• Godlewski, J.; Farhath, M.; Ricklefs, F.L.; Passaro, C.; Kiel, K.; Nakashima, H.; Chiocca, E.A.; Bronisz, A. Oncolytic Virus Therapy Alters the Secretome of Targeted Glioblastoma Cells. Cancers 2021, 13, 1287, doi:10.3390/cancers13061287.
2020
• Bronisz, A.; Rooj, A.K.; Krawczyński, K.; Peruzzi, P.; Salińska, E.; Nakano, I.; Purow, B.; Chiocca, E.A.; Godlewski, J. The Nuclear DICER–Circular RNA Complex Drives the Deregulation of the Glioblastoma Cell MicroRNAome. Science Advaces 2020, 6, eabc0221, doi:10.1126/sciadv.abc0221.
• Krawczynski, K.; Godlewski, J.; Bronisz, A. Oxidative Stress-Part of the Solution or Part of the Problem in the Hypoxic Environment of a Brain Tumor. Antioxidants 2020, 9, 747, doi:10.3390/antiox9080747.
• Bronisz, A.; Salińska, E.; Chiocca, E.A.; Godlewski, J. Hypoxic Roadmap of Glioblastoma-Learning about Directions and Distances in the Brain Tumor Environment. Cancers 2020, 12, 1213, doi:10.3390/cancers12051213.
2019
• Ogawa, D.; Ansari, K.; Nowicki, M.O.; Salińska, E.; Bronisz, A.; Godlewski, J. MicroRNA-451 Inhibits Migration of Glioblastoma While Making It More Susceptible to Conventional Therapy. Non-Coding RNA 2019, 5, 25, doi:10.3390/ncrna5010025.
• Xiao, A.; Brenneman, B.; Floyd, D.; Comeau, L.; Spurio, K.; Olmez, I.; Lee, J.; Nakano, I.; Godlewski, J.; Bronisz, A.; et al. Statins Affect Human Glioblastoma and Other Cancers through TGF-β Inhibition. Oncotarget 2019, 10, 1716–1728, doi:10.18632/oncotarget.26733.
• Godlewski, J.; Lenart, J.; Salinska, E. MicroRNA in Brain Pathology: Neurodegeneration the Other Side of the Brain Cancer. Non-Coding RNA 2019, 5, 20, doi:10.3390/ncrna5010020.
• Bhaskaran, V.; Nowicki, M.O.; Idriss, M.; Jimenez, M.A.; Lugli, G.; Hayes, J.L.; Mahmoud, A.B.; Zane, R.E.; Passaro, C.; Ligon, K.L.; Haas-Kogan, D.; Bronisz, A.; Godlewski, J.; et al. The Functional Synergism of MicroRNA Clustering Provides Therapeutically Relevant Epigenetic Interference in Glioblastoma. Nature Communications 2019, 10, 442, doi:10.1038/s41467-019-08390-z.
2018
• Olmez, I.; Zhang, Y.; Manigat, L.; Benamar, M.; Brenneman, B.; Nakano, I.; Godlewski, J.; Bronisz, A.; Lee, J.; Abbas, T.; et al. Combined C-Met/Trk Inhibition Overcomes Resistance to CDK4/6 Inhibitors in Glioblastoma. Cancer Research 2018, 78, 4360–4369, doi:10.1158/0008-5472.CAN-17-3124.
• Ricklefs, F.L.; Alayo, Q.; Krenzlin, H.; Mahmoud, A.B.; Speranza, M.C.; Nakashima, H.; Hayes, J.L.; Lee, K.; Balaj, L.; Passaro, C.; Rooj, A.K.; Krasemann, S.; Carter, B.S.; Chen, C.C.; Steed, T.; Treiber, J.; Rodig, S.; Yang, K.; Nakano, I.; Lee, H.; Weissleder, R.; Breakefield, X.O.; Godlewski, J.; et al. Immune Evasion Mediated by PD-L1 on Glioblastoma-Derived Extracellular Vesicles. Science Advances 2018, 4, eaar2766, doi:10.1126/sciadv.aar2766.
• Olmez, I.; Love, S.; Xiao, A.; Manigat, L.; Randolph, P.; McKenna, B.D.; Neal, B.P.; Boroda, S.; Li, M.; Brenneman, B.; Abounader, R.; Floyd, D.; Lee, J.; Nakano, I.; Godlewski, J.; et al. Targeting the Mesenchymal Subtype in Glioblastoma and Other Cancers via Inhibition of Diacylglycerol Kinase Alpha. Neuro-Oncology 2018, 20, 192–202, doi:10.1093/neuonc/nox119.
2017
• Olmez, I.; Brenneman, B.; Xiao, A.; Serbulea, V.; Benamar, M.; Zhang, Y.; Manigat, L.; Abbas, T.; Lee, J.; Nakano, I.; Godlewski, J.; et al. Combined CDK4/6 and MTOR Inhibition Is Synergistic against Glioblastoma via Multiple Mechanisms. Clinical Cancer Research 2017, 23, 6958–6968, doi:10.1158/1078-0432.CCR-17-0803.
• Li, M.; Xiao, A.; Floyd, D.; Olmez, I.; Lee, J.; Godlewski, J.; Bronisz, A.; Bhat, K.P.L.; Sulman, E.P.; Nakano, I.; et al. CDK4/6 Inhibition Is More Active against the Glioblastoma Proneural Subtype. Oncotarget 2017, 8, 55319–55331, doi:10.18632/oncotarget.19429.
• Rooj, A.K.; Ricklefs, F.; Mineo, M.; Nakano, I.; Chiocca, E.A.; Bronisz, A.; Godlewski, J. MicroRNA-Mediated Dynamic Bidirectional Shift between the Subclasses of Glioblastoma Stem-like Cells. Cell Reports 2017, 19, 2026–2032, doi:10.1016/j.celrep.2017.05.040.
• Godlewski, J.; Ferrer-Luna, R.; Rooj, A.K.; Mineo, M.; Ricklefs, F.; Takeda, Y.S.; Nowicki, M.O.; Salińska, E.; Nakano, I.; Lee, H.; et al. MicroRNA Signatures and Molecular Subtypes of Glioblastoma: The Role of Extracellular Transfer. Stem Cell Report 2017, 8, 1497–1505, doi:10.1016/j.stemcr.2017.04.024
2016
• Mineo, M.; Ricklefs, F.; Rooj, A.K.; Lyons, S.M.; Ivanov, P.; Ansari, K.I.; Nakano, I.; Chiocca, E.A.; Godlewski, J.; Bronisz, A. The Long Non-Coding RNA HIF1A-AS2 Facilitates the Maintenance of Mesenchymal Glioblastoma Stem-like Cells in Hypoxic Niches. Cell Reports 2016, 15, 2500–2509, doi:10.1016/j.celrep.2016.05.018.
• Ricklefs, F.; Mineo, M.; Rooj, A.K.; Nakano, I.; Charest, A.; Weissleder, R.; Breakefield, X.O.; Chiocca, E.A.; Godlewski, J.; Bronisz, A. Extracellular Vesicles from High-Grade Glioma Exchange Diverse Pro-Oncogenic Signals That Maintain Intratumoral Heterogeneity. Cancer Research 2016, 76, 2876–2881, doi:10.1158/0008-5472.CAN-15-3432.
• Rooj, A.K.; Bronisz, A.; Godlewski, J. The Role of Octamer Binding Transcription Factors in Glioblastoma Multiforme. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms 2016, 1859, 805–811, doi:10.1016/j.bbagrm.2016.03.003.
• Teplyuk, N.M.; Uhlmann, E.J.; Gabriely, G.; Volfovsky, N.; Wang, Y.; Teng, J.; Karmali, P.; Marcusson, E.; Peter, P.; Mohan, A.; Kraytsberg, Y.; Cialic, R.; Chiocca, E.A.; Godlewski, J.; et al. Therapeutic Potential of Targeting MicroRNA-10b in Established Intracranial Glioblastoma: First Steps toward the Clinic. EMBO Molecular Medicine 2016, 8, 268–287, doi:10.15252/emmm.201505495.
• Bronisz, A.; Godlewski, J.; Chiocca, E.A. Extracellular Vesicles and MicroRNAs: Their Role in Tumorigenicity and Therapy for Brain Tumors. Cellular and Molecular Neurobiology 2016, 36, 361–376, doi:10.1007/s10571-015-0293-4.
• Rooj, A.K.; Mineo, M.; Godlewski, J. MicroRNA and Extracellular Vesicles in Glioblastoma – Small but Powerful. Brain Tumor Patholology 2016, 33, 77–88, doi:10.1007/s10014-016-0259-3.
2015
• Ansari, K.I.; Ogawa, D.; Rooj, A.K.; Lawler, S.E.; Krichevsky, A.M.; Johnson, M.D.; Chiocca, E.A.; Bronisz, A.; Godlewski, J. Glucose-Based Regulation of MiR-451/AMPK Signaling Depends on the OCT1 Transcription Factor. Cell Reports 2015, 11, 902–909, doi:10.1016/j.celrep.2015.04.016.
• Bronisz, A.; Chiocca, E.A.; Godlewski, J. Response to Energy Depletion: MiR-451/AMPK Loop. Oncotarget 2015, 6, 17851–17852.
Godlewski, J.; Krichevsky, A.M.; Johnson, M.D.; Chiocca, E.A.; Bronisz, A. Belonging to a Network-MicroRNAs, Extracellular Vesicles, and the Glioblastoma Microenvironment. Neuro-Oncology 2015, 17, 652–662, doi:10.1093/neuonc/nou292.
2014
• Bronisz, A.; Wang, Y.; Nowicki, M.O.; Peruzzi, P.; Ansari, K.I.; Ogawa, D.; Balaj, L.; Rienzo, G.D.; Mineo, M.; Nakano, I.; Ostrowski, M.C.; Hochberg, F.; Weissleder, R.; Lawler, S.E., Chiocca, E.A.; Godlewski, J. Extracellular Vesicles Modulate the Glioblastoma Microenvironment via a Tumor Suppression Signaling Network Directed by MiR-1. Cancer Research 2014, 74, 738–750, doi:10.1158/0008-5472.CAN-13-2650.
2013
• Peruzzi, P.; Bronisz, A.; Nowicki, M.O.; Wang, Y.; Ogawa, D.; Price, R.; Nakano, I.; Kwon, C.-H.; Hayes, J.; Lawler, S.E.; Ostrowski, M.C.; Chiocca, E.A.; Godlewski, J. MicroRNA-128 Coordinately Targets Polycomb Repressor Complexes in Glioma Stem Cells. Neuro-Oncology 2013, 15, 1212–1224, doi:10.1093/neuonc/not055.
• Guessous, F.; Alvarado-Velez, M.; Marcinkiewicz, L.; Zhang, Y.; Kim, J.; Heister, S.; Kefas, B.; Godlewski, J.; Schiff, D.; Purow, B.; et al. Oncogenic Effects of MiR-10b in Glioblastoma Stem Cells. Journal of Neurooncology 2013, 112, 153–163, doi:10.1007/s11060-013-1047-0.
2012
• Bronisz, A.; Godlewski, J.; Wallace, J.A.; Merchant, A.S.; Nowicki, M.O.; Mathsyaraja, H.; Srinivasan, R.; Trimboli, A.J.; Martin, C.K.; Li, F.; et al. Reprogramming of the Tumour Microenvironment by Stromal PTEN-Regulated MiR-320. Nature Cell Biology 2012, 14, 159–167, doi:10.1038/ncb2396.
2011
• Williams, S.P.; Nowicki, M.O.; Liu, F.; Press, R.; Godlewski, J.; Abdel-Rasoul, M.; Kaur, B.; Fernandez, S.A.; Chiocca, E.A.; Lawler, S.E. Indirubins Decrease Glioma Invasion by Blocking Migratory Phenotypes in Both the Tumor and Stromal Endothelial Cell Compartments. Cancer Research 2011, 71, 5374–5380, doi:10.1158/0008-5472.CAN-10-3026.
• Guerau-de-Arellano, M.; Smith, K.M.; Godlewski, J.; Liu, Y.; Winger, R.; Lawler, S.E.; Whitacre, C.C.; Racke, M.K.; Lovett-Racke, A.E. Micro-RNA Dysregulation in Multiple Sclerosis Favours pro-Inflammatory T-Cell-Mediated Autoimmunity. Brain 2011, 134, 3578–3589, doi:10.1093/brain/awr262.
