Head
Anna Sarnowska, MD, PhD, DSc, phone: +48 22 60 86 598, This email address is being protected from spambots. You need JavaScript enabled to view it.,
Secretary
Dorota Czajkowska, MSc, phone: +48 22 60 86 475, This email address is being protected from spambots. You need JavaScript enabled to view it. 
Research staff
Aleksandra Bzinkowska, MSc; phone: +48 22 60 86 463, This email address is being protected from spambots. You need JavaScript enabled to view it.
Magdalena Chodkowska, MSc; phone: +48 22 60 86 463, This email address is being protected from spambots. You need JavaScript enabled to view it.
Klaudia Radoszkiewicz, MSc; phone: +48 22 60 86 463, This email address is being protected from spambots. You need JavaScript enabled to view it. 
Paulina Rybkowska, MSc; phone: +48 22 60 86 463, This email address is being protected from spambots. You need JavaScript enabled to view it. 
Agnieszka Smolińska, MSc; phone: +48 22 60 86 463, This email address is being protected from spambots. You need JavaScript enabled to view it.
PhD students
Monika Sypecka, MSc, tel. 22 60 86 463, This email address is being protected from spambots. You need JavaScript enabled to view it.
Trainees
Katarzyna Marcinkowska 
Maja Pelczarska 
Aleksandra Pobudkiewicz 
Ilona Zembrzuska-Kaska

Specialist Team Tissue and Cell Bank + Advanced therapy medicinal product manufacture
Aleksandra Dominiak M.Sc., phone: 22 60 86 637; This email address is being protected from spambots. You need JavaScript enabled to view it.
Katarzyna Gąsowska M.Sc., phone 22 60 86 504; This email address is being protected from spambots. You need JavaScript enabled to view it.
Daniel Szopa M.Sc., phone: 22 60 86 504; This email address is being protected from spambots. You need JavaScript enabled to view it.
Katarzyna Jezierska-Woźniak Ph.D., phone 89 524 53 38,  This email address is being protected from spambots. You need JavaScript enabled to view it.

Research / clinic profile

The Translational Research Platform in the field of regenerative medicine conducts translational research at the preclinical and clinical level.

 Tissue and Cell Bank - The Minister for Health approval for
• collection, processing and storage of subcutaneous fat,
• collection, processing, storage and release of umbilical cord,
• collection, processing, storage, release and distribution of mesenchymal cells isolated from adipose tissue,
• collection, processing, storage, release and distribution of mesenchymal cells isolated from Wharton's Jelly in clinical application

• pilot studies in cell therapy - establishing and organizing cooperation between scientists and medical doctors in clinics, regenerative potential cells in clinical application
• developing a new treatment and therapeutic strategies using various (source) types of stem cells (mesenchymal stem cells, neural stem cells)
• experimental regenerative therapy for patients with amyotrophic lateral sclerosis ALS using (ADRC-adipose derived regenerative cells) isolated from adipose tissue, establishing additional tests necessary to assess the safety and effectiveness of the therapy, estimating the costs of therapy – studies in cooperation with the Department of Neurosurgery and Neurology of the Medical University of Warsaw
• development of criteria for the inclusion of patients with autoimmune drug-resistant epilepsy to experimental regenerative therapy with the use of ADRC, additional tests necessary to assess the safety and effectiveness of the therapy used - in cooperation with the Department of Neurology and the Department of Surgery of the Institute of Mother and Child in Warsaw
• study for clinical application of ADRC cells in non-healing wound treatment) and peripheral nerves damages after failure reconstructions in the past - in cooperation with the Plastic Surgery Clinic of Centre of Postgraduate Medical Education (CMKP)
• assessment of the regenerative potential of mesenchymal stem cells derived from Wharton's jelly placed on 3D skeletons in the reconstruction of the alveolar ridge in children with a cleft defect - in cooperation with the Department of Children's and Adolescent Surgery of the Institute of Mother and Child

Grants
• “Bilateral interactions between WJ-MSC and fNSC affecting the niche formation and regenerative abilities” NCN -Opus 16 nr 2018/31/B/NZ4/03172; Supervisor: dr hab. Anna Sarnowska; Term: - 2019-2022

• “Activity and function of auxiliary glucose metabolic fluxes in response to low oxygen (physioxia) during long-term AD-MSC culture” NCN - Preludium 16 nr 2018/31/N/NZ4/03275; PI: mgr Anna Sylwia Figiel-Dąbrowska; Term: - 2019-2022

• „Innovative application of stem cells and biocompatible polyelectrolyte nanoparticles releasing neurotrophins in adjuvant treatment of neurodegenerative diseases”; PI: dr hab. Anna Sarnowska  Term: – 2021-2025

• „Development of an innovative advanced therapy medicinal product based on the mesenchymal stem cells of Wharton's jelly in amyotrophic lateral sclerosis treatmen”. Contract with Polish Stem Cell Bank: „Perform preclinical studies of the advanced therapy medicinal product”.
PI: dr hab. Anna Sarnowska (Zadanie 1), prof. dr hab. Krystyna Domanska-Janik (Zadanie2): prof. dr hab. Leonora Bużańska (Zadanie 3 i 4) Term: 2018-2022.

Collaboration
domestic
• Department of Neurology Medical University, Warsaw
• Child and Adolescent Neurology Child Institute of Mother and Child, Warsaw
• Department of Paediatric Surgery Institute of Mother and Child , Warsaw
• CMKP Department of Plastic Surgery, Warsaw
• Polish Stem Cell Bank, Warsaw (order contract finished in 2021)

foreign
• Università degli Studi di Milano-Bicocca, UNIMIB,Department of Biotechnology and Biosciences, Milan, Italy
• Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan
• Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Zagreb, Croatia
• Departments of Physical Medicine & Rehabilitation and Neurosurgery, Harvard Medical School, Spaulding Rehabilitation Hospital and Brigham and Women's Hospital, Boston, MA, USA

Research equipment
• Tissue and Cell Bank – clean room with fully equipped cell isalation, culture and storage
• Cell culture research laboratory
• AutoMACS Pro Separator, Miltenyibiotec, USA
• Cytoria, Celution

Research methods
• in vitro cell culture, mesenchymal stem cells from multiple sources: adipose tissue, umbilical cord, trabecular bone – isolation and primary culture,
• endothelial cells - derivation from mesenchymal stem cells,
• neural stem cells – isolation and primary culture,
• organotypic hippocampal ex vivo culture
• oxygen-glucose deprivation (OGD) model of ischemic hippocampal injury,
• functional tests: neuroprotective and migration potential of cells
• immunocytochemistry stainings
• flow cytometry
• magnetic activated cell sorting – AutoMACS Pro Separation
• molecular biology techniques– qPCR, Western Blot, ELISA

Selected publications

• Sypecka M, Bzinkowska A, Sulejczak D, Dabrowski F, Sarnowska A. Evaluation of the optimal manufacturing protocols and therapeutic properties of mesenchymal stem/stromal cells derived from Wharton’s Jelly. Int J Mol Sci. 2023;24,652:1-21.doi:10.3390/ijms24010652. (Oryginalna, open acces). IF = 5,6
• Smolinska A, Bzinkowska A, Rybkowska P, Chodkowska M, Sarnowska A. Promising markers in the context of mesenchymal stem/stromal cells subpopulations with unique properties. Stem Cells Int. 2023 Sep 20;2023:1842958. doi: 10.1155/2023/1842958. eCollection 2023. (Review, open acces). IF = 4,3
• Radoszkiewicz K, Hribljan V, Isakovic J, Mitrecic D, Sarnowska A. Critical points for optimizing long-term culture and neural differentiation capacity of rodent and human neural stem cells to facilitate translation into clinical settings. Exp Neurol. 2023 May;363:114353. doi: 10.1016/j.expneurol.2023.114353. Epub 2023 Feb 24. PMID: 36841464. (Review, open acces). IF = 5,3
• Radoszkiewicz K, Jezierska-Woźniak K, Waśniewski T, Sarnowska A. Understanding intra- and inter-species variability in neural stem cells' biology is key to their successful cryopreservation, culture, and propagation. Cells. 2023 Feb 2;12(3):488. doi: 10.3390/cells12030488. (Oryginalna, open acces). IF = 6,0
• Rybkowska P, Radoszkiewicz K, Kawalec M, Dymkowska D, Zabłocka B, Zabłocki K, Sarnowska A. The metabolic changes between monolayer (2D) and three-dimensional (3D) culture conditions in human mesenchymal stem/stromal cells derived from adipose tissue. Cells. 2023 Jan 1;12(1):178. doi: 10.3390/cells12010178. (Oryginalna, open acces). IF = 6,0
• Farzaneh M, Nasrolahi A, Ghaedrahmati F, Masoodi T, Najafi S, Sheykhi-Sabzehpoush M, Dari MAG, Radoszkiewicz K, Uddin S, Azizidoost S, Khoshnam SE Potential roles of lncRNA-XIST/miRNAs/mRNAs in human cancer cells. Clin Transl Oncol. 2023 Jul;25(7):2015-2042. doi: 10.1007/s12094-023-03110-y. (Review, open acces). IF = 3,4
• Nasrolahi A, Azizidoost S, Radoszkiewicz K, Najafi S, Ghaedrahmati F, Sheykhi-Sabzehpoush M, Poodineh J, Hajizadeh M, Anbiyaiee A, Farzaneh M, Feghhi M. Long non-coding RNAs involved in retinoblastoma. J Cancer Res Clin Oncol. 2023 Jan;149(1):401-421. doi: 10.1007/s00432-022-04398-z. (Review, open acces). IF = 3,6
• Nasrolahi A, Azizidoost S, Radoszkiewicz K, Najafi S, Ghaedrahmati F, Anbiyaee O, Khoshnam SE, Farzaneh M, Uddin S. Signaling pathways governing glioma cancer stem cells behavior. Cell Signal. 2023 Jan;101:110493. doi: 10.1016/j.cellsig.2022.110493. (Review, open acces). IF = 4,8
• Farzaneh M, Masoodi T, Ghaedrahmati F, Radoszkiewicz K, Anbiyaiee A, Sheykhi-Sabzehpoush M, Rad NK, Uddin S, Jooybari SPM, Khoshnam SE, Azizidoost S. An updated review of contribution of long noncoding RNA-NEAT1 to the progression of human cancers. Pathol Res Pract. 2023 May;245:154380. doi: 10.1016/j.prp.2023.154380. IF= 2,8
• Natalia E. Krzesniak, Anna Sarnowska, Anna Figiel-Dabrowska, Katarzyna Osiak, Krystyna Domanska-Janik, Bartłomiej H Noszczyk, 2021: Secondary release of the peripheral nerve with autologous fat derivates benefits for functional and sensory recovery, Neural Regeneration Research, https://doi.org/10.4103/1673-5374.297081
• Anna Figiel-Dabrowska, Klaudia Radoszkiewicz, Paulina Rybkowska, Natalia Ewa Krzesniak, Dorota Sulejczak, Anna Sarnowska, 2021: Neurogenic and Neuroprotective Potential of Stem/Stromal Cells Derived from Adipose Tissue, Cells, https://doi.org/10.3390/cells10061475
• Aleksandra Wedzinska, Anna Figiel-Dabrowska, Hanna Kozlowska, Anna Sarnowska, 2021: The Effect of Proinflammatory Cytokines on the Proliferation, Migration and Secretory Activity of Mesenchymal Stem/Stromal Cells (WJ-MSCs) under 5% O 2 and 21% O 2 Culture Conditions, Journal of Clinical Medicine, https://doi.org/10.3390/jcm10091813
• Agnieszka Kamińska, Aleksandra Wedzinska, Marta Kot, Anna Sarnowska, 2021. Effect of Long-Term 3D Spheroid Culture on WJ-MSC. Cells https://doi.org/10.3390/cells10040719
• Michal Kosinski, Anna Figiel-Dabrowska, Wioletta Lech, Lukasz Wieprzowski, Ryszard Strzalkowski, Damian Strzemecki, Lukasz Cheda, Jacek Lenart, Krystyna Domanska-Janik and Anna Sarnowska, 2020: Bone Defect Repair Using a Bone Substitute Supported by Mesenchymal Stem Cells Derived from the Umbilical Cord, Stem Cells International, https://doi.org/10.1155/2020/1321283
• Elzbieta Szczepanik, Hanna Mierzewska, Dorota Antczak-Marach, Anna Figiel-Dabrowska, Iwona Terczynska, Jolanta Tryfon, Natalia Krzesniak, Bartlomiej Henryk Noszczyk, Ewa Sawicka, Krystyna Domanska-Janik, Anna Sarnowska, 2020: Intrathecal Infusion of Autologous Adipose-Derived Regenerative Cells in Autoimmune Refractory Epilepsy: Evaluation of Safety and Efficacy, Stem Cells International, https://doi.org/10.1155/2020/7104243
• Talita Glaser, Hiromi Shimojo, Deidiane Elisa Ribeiro, Patrícia Pereira Lopes Martins, Renata Pereira Beco , Michal Kosinski, Vanessa Fernandes Arnaud Sampaio, Juliana Corrêa-Velloso 1, Ágatha Oliveira-Giacomelli, Claudiana Lameu, Ana Paula de Jesus Santos , Héllio Danny Nóbrega de Souza , Yang D Teng , Ryoichiro Kageyama , Henning Ulrich, 2020: ATP and spontaneous calcium oscillations control neural stem cell fate determination in Huntington's disease: a novel approach for cell clock research, Mol Psychiatry, https://doi: 10.1038/s41380-020-0717-5
• Letizia Mazzini,Daniela Ferrari,Pavle R Andjus, Leonora Buzanska, Roberto Cantello, Fabiola De Marchi, Maurizio Gelati, Rashid Giniatullin, Joel C. Glover, Mariagrazia Grilli, Elena N. Kozlova, Margherita Maioli, Dinko Mitrečić, Augustas Pivoriunas, Rosario Sanchez-Pernaute, Anna Sarnowska, Angelo L. Vescovi, 2018: Advances in stem cell therapy for amyotrophic lateral sclerosis, Expert Opinion on Biological Therapy, https://doi.org/10.1080/14712598.2018.1503248
• Sylwia Dabrowska, Joanna Sypecka, Anna Jablonska, Lukasz Strojek, Miroslaw Wielgos, Krystyna Domanska-Janik, Anna Sarnowska; 2017: Neuroprotective Potential and Paracrine Activity of Stromal Vs. Culture-Expanded hMSC Derived from Wharton Jelly under Co-Cultured with Hippocampal Organotypic Slices, Molecular Neurobiology, https://doi.org/10.1007/s12035-017-0802-1
• Wioletta Lech, Anna Figiel-Dabrowska, Anna Sarnowska, Katarzyna Drela, Patrycja Obtulowicz, Bartlomiej Henryk Noszczyk, Leonora Buzanska,Krystyna Domanska-Janik, 2016: Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy, Stem Cell International, https://doi.org/10.1155/2016/2514917
• Patrycja Obtulowicz, Wioletta Lech, Lukasz Strojek, Anna Sarnowska, Krystyna Domanska-Janik, 2016: Induction of Endothelial Phenotype from Wharton's Jelly-Derived MSCs and Comparison of Their Vasoprotective and Neuroprotective Potential with Primary WJ-MSCs in CA1 Hippocampal Region Ex Vivo, Molecular Neurobiology, https://doi.org/10.1007/s12035-017-0802-1